Product nameAnti-Neurocan antibody [EPR6397]
See all Neurocan primary antibodies
DescriptionRabbit monoclonal [EPR6397] to Neurocan
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Neurocan aa 1-100. The exact sequence is proprietary.
- Human hippocampus and hypothalamus lysates; Human brain tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain.
- Information by UniProt
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
All lanes : Anti-Neurocan antibody [EPR6397] (ab125021) at 1/10000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 130,270 kDa why is the actual band size different from the predicted?
ab125021, at 1/250 dilution, staining Neurocan in paraffin-embedded Human brain tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
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