Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
Key features and details
- Mouse monoclonal [3H8] to NeuroD1 - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-NeuroD1 antibody [3H8] - BSA and Azide free
See all NeuroD1 primary antibodies -
Description
Mouse monoclonal [3H8] to NeuroD1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Sheep, Cow -
Immunogen
Recombinant fragment with tag: QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT , corresponding to amino acids 201-300 of Human NeuroD1
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Positive control
- IMR-32 (human neuroblastoma) whole cell lysate and human ovary, clear cell carcinoma tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3H8 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (5) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. -
Involvement in disease
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4760 Human
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
- Unigene: 709709 Human
- Unigene: 44289 Rat
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Alternative names
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
Images
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Immunohistochemical analysis of formaldehyde fixed human cerebellum sections incubated with ab60704 for 20 minutes at 25°C in a concentration of 1/400. The blocking step was performed with 3% H2O2 for 10 minutes at 25ºC. The secondary antibody used was a polyclonal goat anti-mouse/rabbit HRP conjugate, used undiluted.
This image was generated using the ascites version of the product.
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Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704) + IMR-32 (Human Caucasian neuroblastoma) whole cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
This image was generated using the ascites version of the product.
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Overlay histogram showing SHSY-5Y cells stained with ab60704 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60704, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (51)
ab60704 has been referenced in 51 publications.
- Anastasaki C et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc 3:101173 (2022). PubMed: 35199037
- Li T et al. BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging. Biomedicines 10:N/A (2022). PubMed: 35625884
- Tian Y et al. Single-cell transcriptomic profiling reveals the tumor heterogeneity of small-cell lung cancer. Signal Transduct Target Ther 7:346 (2022). PubMed: 36195615
- Ke X et al. Adverse Maternal Environment Alters MicroRNA-10b-5p Expression and Its Epigenetic Profile Concurrently with Impaired Hippocampal Neurogenesis in Male Mouse Hippocampus. Dev Neurosci 43:95-105 (2021). PubMed: 33940573
- Pebworth MP et al. Human intermediate progenitor diversity during cortical development. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34155100