Recombinant
RabMAb

Recombinant Anti-NeuroD1 antibody [EPR20766] (ab213725)

Overview

  • Product name

    Anti-NeuroD1 antibody [EPR20766]
    See all NeuroD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20766] to NeuroD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NeuroD1 aa 50-300. The exact sequence is proprietary.
    Database link: Q13562

  • Positive control

    • WB: Y79 whole cell lysate; rat retina tissue lysate and mouse P3 (postnatal day 3) retina tissue lysate. IP: Y79 whole cell lysate. IHC-P: Mouse hippocampus tissue; rat hippocampus tissue. Flow cytometry: Y97 cells.
  • General notes

    The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

Flow Cyt 1/50.
IP 1/30.

Target

  • Function

    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • Involvement in disease

    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1/1000 dilution

    Lane 1 : Y79 (human retinoblastoma cell line) whole cell lysate
    Lane 2 : Mouse P3 (postnatal day 3) retina lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST

    Exposure times.

    Lane 1: 10 seconds

    Lane 2: 30 seconds

  • Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1000 cells + Rat retina tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

    Lane 1: Y79 whole cell lysate 10 μg (Input).
    Lane 2: ab213725 IP in Y79 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213725 in Y79 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab213725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat, Primary cortical mixed neuronal culture)
Permeabilization
No
Specification
Rat, Primary cortical mixed neuronal culture
Blocking step
3%goat serum in 0.1% Triton in PBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Tingting Wang

Verified customer

Submitted Aug 13 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 mouse embryo)
Permeabilization
No
Specification
E12.5 mouse embryo
Blocking step
TSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 17 2018

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