Recombinant
RabMAb

Recombinant Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)

Overview

  • Product name

    Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free
    See all NeuroD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20766] to NeuroD1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NeuroD1 aa 50-300. The exact sequence is proprietary.
    Database link: Q13562

  • Positive control

    • IHC-P: Mouse hippocampus tissue.
  • General notes

    ab226489 is the carrier-free version of ab213725 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab226489 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • Involvement in disease

    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213725).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Y79 whole cell lysate 10 μg (Input).
    Lane 2: ab213725 IP in Y79 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213725 in Y79 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213725).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213725).

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213725).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab226489 has not yet been referenced specifically in any publications.

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