RecombinantRabMAb

Recombinant Anti-NeuroD1 antibody [EPR4008] (ab109224)

Reviews (1) Submit a question References (7)
Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
  • Immunocytochemistry - Anti-NeuroD1 antibody [EPR4008] (ab109224)
  • Anti-NeuroD1 antibody [EPR4008] (ab109224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4008] to NeuroD1
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NeuroD1 antibody [EPR4008]
    See all NeuroD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4008] to NeuroD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Y79 and fetal brain lysates; ICC: Mouse primary neuron cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/200.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
Notes
ICC
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.

    • Involvement in disease

      Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.

    • Post-translational
      modifications

      Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • atonal antibody
      • basic helix loop helix transcription factor antibody
      • BETA 2 antibody
      • Beta cell E box transactivator 2 antibody
      • BETA2 antibody
      • BHF 1 antibody
      • BHF1 antibody
      • bHLHa3 antibody
      • class A basic helix loop helix protein 3 antibody
      • Class A basic helix-loop-helix protein 3 antibody
      • MODY 6 antibody
      • MODY6 antibody
      • NDF1_HUMAN antibody
      • NeuroD antibody
      • NeuroD1 antibody
      • Neurogenic differentiation 1 antibody
      • Neurogenic differentiation factor 1 antibody
      • neurogenic helix loop helix protein NEUROD antibody
      • Neuronal differentiation 1 antibody
      see all

    Images

    • Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
      Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
      All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

      Lane 1 : Y79 lysates
      Lane 2 : Fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry - Anti-NeuroD1 antibody [EPR4008] (ab109224)
      Immunocytochemistry - Anti-NeuroD1 antibody [EPR4008] (ab109224)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    • Anti-NeuroD1 antibody [EPR4008] (ab109224)
      Anti-NeuroD1 antibody [EPR4008] (ab109224)

    References (7)

    Publishing research using ab109224? Please let us know so that we can cite the reference in this datasheet.

    ab109224 has been referenced in 7 publications.

    • Li HJ  et al. Intestinal Neurod1 expression impairs paneth cell differentiation and promotes enteroendocrine lineage specification. Sci Rep 9:19489 (2019). PubMed: 31862906
    • Wölnerhanssen BK  et al. Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals. Sci Rep 7:8174 (2017). PubMed: 28811552
    • Mollaoglu G  et al. MYC Drives Progression of Small Cell Lung Cancer to a Variant Neuroendocrine Subtype with Vulnerability to Aurora Kinase Inhibition. Cancer Cell 31:270-285 (2017). IHC-P . PubMed: 28089889
    • Zhang F  et al. Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. Neurosci Bull 32:205-16 (2016). PubMed: 27146165
    • Borromeo MD  et al. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs. Cell Rep 16:1259-72 (2016). WB ; Human . PubMed: 27452466
    • Hunter CS & Stein R Characterization of an apparently novel ß-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. J Biol Chem 288:3795-803 (2013). EMSA ; Mouse . PubMed: 23269676
    • Hunter CS  et al. Islet a-, ß-, and d-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. Diabetes 62:875-86 (2013). IP . PubMed: 23193182

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-NeuroD1 antibody [EPR4008]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (10% SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (Lung cancer cells)
    Specification
    Lung cancer cells
    Blocking step
    Odyssey Blocking Buffer in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com