Product nameAnti-NeuroD1 antibody [EPR4008]
See all NeuroD1 primary antibodies
DescriptionRabbit monoclonal [EPR4008] to NeuroD1
Tested applicationsSuitable for: EMSA, WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NeuroD1. The exact sequence is proprietary.
- Y79 and fetal brain lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent concentration. PubMed: 23269676|
|WB||1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).|
|IP||1/10 - 1/100.|
FunctionDifferentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
Involvement in diseaseDefects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution
Lane 1 : Y79 lysates
Lane 2 : Fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Wölnerhanssen BK et al. Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals. Sci Rep 7:8174 (2017). Read more (PubMed: 28811552) »
- Mollaoglu G et al. MYC Drives Progression of Small Cell Lung Cancer to a Variant Neuroendocrine Subtype with Vulnerability to Aurora Kinase Inhibition. Cancer Cell 31:270-285 (2017). IHC-P . Read more (PubMed: 28089889) »