Recombinant Anti-NeuroD1 antibody [EPR4008] (ab109224)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4008] to NeuroD1
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-NeuroD1 antibody [EPR4008]
See all NeuroD1 primary antibodies -
Description
Rabbit monoclonal [EPR4008] to NeuroD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Y79 and fetal brain lysates; ICC: Mouse primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4008 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
1/200.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
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Notes |
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ICC
1/200. |
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. -
Involvement in disease
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4760 Human
- Entrez Gene: 18012 Mouse
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q60867 Mouse
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
see all -
Alternative names
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
Images
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All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution
Lane 1 : Y79 lysates
Lane 2 : Fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109224 has been referenced in 7 publications.
- Li HJ et al. Intestinal Neurod1 expression impairs paneth cell differentiation and promotes enteroendocrine lineage specification. Sci Rep 9:19489 (2019). PubMed: 31862906
- Wölnerhanssen BK et al. Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals. Sci Rep 7:8174 (2017). PubMed: 28811552
- Mollaoglu G et al. MYC Drives Progression of Small Cell Lung Cancer to a Variant Neuroendocrine Subtype with Vulnerability to Aurora Kinase Inhibition. Cancer Cell 31:270-285 (2017). IHC-P . PubMed: 28089889
- Zhang F et al. Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. Neurosci Bull 32:205-16 (2016). PubMed: 27146165
- Borromeo MD et al. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs. Cell Rep 16:1259-72 (2016). WB ; Human . PubMed: 27452466
- Hunter CS & Stein R Characterization of an apparently novel ß-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. J Biol Chem 288:3795-803 (2013). EMSA ; Mouse . PubMed: 23269676
- Hunter CS et al. Islet a-, ß-, and d-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. Diabetes 62:875-86 (2013). IP . PubMed: 23193182