Mouse Tissue lysate - whole (Cerebral cortex tissue from postnatal day 0 mouse)
Cerebral cortex tissue from postnatal day 0 mouse
ChIP performed with unrelated antibody (anti-GFP antibody).
Cross-linking (X-ChIP) Duration of cross-linking step: 10 minute(s) and 0 second(s) Specification of the cross-linking agent: 1% formaldehyde
Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.
Primer sequence for PCR was:
Other product details
2 hour(s) and 0 minute(s) · Temperature: 4°C
We are very happy that this antibody provided good results for this customer.
Since the customer obtained positive results in ChiP, we decided to re-test this antibody in IP to determine its suitability for this application.
The lab re-tested the same lot used by the customer in his ChiP assay. Unfortunately, the results obtained were negative therefore we still do not recommend this antibody in IP.
Please feel free to contact our Scientific Support team if you have some feedback you would like to share and/or if you have any question in this matter.
Abcam user community
Submitted Apr 18 2014
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