Recombinant Anti-NeuroD2 antibody [EPR5135] (ab109406)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5135] to NeuroD2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-NeuroD2 antibody [EPR5135]
See all NeuroD2 primary antibodies -
Description
Rabbit monoclonal [EPR5135] to NeuroD2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human cerebellum, Human hippocampus, and fetal brain, Mouse cerebellum, Rat cerebellum lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5135 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa). |
Target
-
Function
Appears to mediate neuronal differentiation. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4761 Human
- Entrez Gene: 18013 Mouse
- Entrez Gene: 54276 Rat
- Omim: 601725 Human
- SwissProt: Q15784 Human
- SwissProt: Q62414 Mouse
- SwissProt: Q63689 Rat
- Unigene: 322431 Human
see all -
Alternative names
- bHLHa1 antibody
- class A basic helix loop helix protein 1 antibody
- Class A basic helix-loop-helix protein 1 antibody
see all
Images
-
All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution (Purified)
Lane 1 : Mouse cerebellum lysates
Lane 2 : Rat cerebellum lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution (Purified)
Lane 1 : Human cerebellum tissue lysate at 20 µg
Lane 2 : Human fetal brain tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human hippocampus lysate
Lane 3 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab109406 has been referenced in 8 publications.
- Lipinski M et al. KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Nat Commun 11:2588 (2020). PubMed: 32444594
- Guzelsoy G et al. Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2. Sci Rep 9:19697 (2019). PubMed: 31873146
- van Weert LTCM et al. Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling. Int J Mol Sci 20:N/A (2019). PubMed: 30934833
- Peter CJ et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun 10:4112 (2019). PubMed: 31511512
- Hahn MA et al. Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation. Sci Adv 5:eaax0080 (2019). PubMed: 31681843
- Bayam E et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . PubMed: 26341353
- Katsushima K et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . PubMed: 22736761
- Guner G et al. NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons. eNeuro 4:N/A (0). PubMed: 28303257