Overview

  • Product name

    Anti-NEUROD4 antibody
  • Description

    Rabbit polyclonal to NEUROD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: HMETHLLHLK PQVFKSLGES SFGSHLPDCS TPPYEGPLTP PLSISGNFSL, corresponding to internal amino acids 216 - 265 of Human NEUROD4 (NP_067014).

  • Positive control

    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-NEUROD4 antibody (ab90484) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 37 kDa



    Gel concentration 12%

References

ab90484 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for your inquiry. Indeed, it is difficult to find antibodies crossreactive with Zebrafish. I don't know whether I have already told you about our list of Zebrafish crossreactive antibodies? It can be found under the following link: www.abcam.com/zebrafish In regards to choosing the most probably crossreacting antibody for your three targets of interest, please find my comments here below. As for the previous antibody, please do not hesitate to ask for the discount code for testing these antibodies on Zebrafish (www.abcam.com/collaborationdiscount) I would like also to recommend, to chose antibodies which have been tested in the application or at least a similar application to which you will be using. Antibodies against Neurogenin 1: The ab89461 has a good alignment score and it seems likely that crossreactivity could happen. The score is 68% http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-113055-0569-24134208-pg https://www.abcam.com/index.html?datasheet=89461 Also the ab117847 has reasonable chances to detect the Zebrafish protein, as the full length human protein has been used as an immunogen and the score is 47%. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-105747-0570-80392552-pg https://www.abcam.com/index.html?datasheet=117847 The two other antibodies, ab66498 and ab71818, are generated against peptides from N and C term, and as the homology there is not very good with the Zebrafish protein, I would recommend to test the other two antibodies first. NeuroD4 The immunogen of ab90484 has stretch conserved and I think it could work therefore. The score is 50% and the alignment can be found here: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-110656-0883-28043163-pg https://www.abcam.com/index.html?datasheet=90484 The ab67168 has been produced with the full length human protein and the overall score is with 55% also relatively ok. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-110901-0500-99792871-pg https://www.abcam.com/index.html?datasheet=67168 Ephrin B3 The overall protein alignment between the human and Zebrafish protein is relatively good, with a score of 54%.http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-111305-0838-36391612-pg The ab101699 has a reasonable good alignement and I could recommend to test this antibody therefore. The alignment is ********:::***: with a score of 73% http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-111510-0668-3570069-pg https://www.abcam.com/index.html?datasheet=101699 Of the other antibodies, I can also recommend to test either the ab28389 or the ab89474, as they have been produced with relatively long sequences and possibility of crossreactivity is therefore good as well. https://www.abcam.com/index.html?datasheet=28389 https://www.abcam.com/index.html?datasheet=89474 I would not recommend the ab53063 and for the ab103001 and ab106344 I do not know the immunogen sequences. If however these antibodies would interest you also in regards to their tested applications, I will be happy to ask the laboratory for prediction of the crossreaction. I hope this information is helpful. Please do not hesitate to contact me for the discount codes or for further information.

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