Overview

  • Product name

    Anti-Neurofascin antibody [EPR19003]
    See all Neurofascin primary antibodies
  • Description

    Rabbit monoclonal [EPR19003] to Neurofascin
  • Host species

    Rabbit
  • Specificity

    IHC is recommended for mouse and rat only.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Neurofascin aa 1-350. The exact sequence is proprietary.
    Database link: Q810U3

  • Positive control

    • WB: Human brain and cerebellum lysates; Mouse and rat brain and spinal cord lysates. IHC-P: Mouse cerebrum and colon tissues. Rat cerebrum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 186, 155, 125, 75 kDa (predicted molecular weight: 138 kDa).

Target

Images

  • All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

    Lane 1 : Human brain lysate at 20 µg
    Lane 2 : Human cerebellum lysate at 20 µg
    Lane 3 : Human lung lysate at 20 µg
    Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 138 kDa
    Observed band size: 125,155,186,75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).

    The 75 kDa band represents one of the isoforms annotated in UniProt.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse cerebrum is observed [PMID: 23198679].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse spinal cord lysate
    Lane 4 : Rat spinal cord lysate
    Lane 5 : Mouse lung lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate
    Lane 8 : Rat kidney lysate
    Lane 9 : Rat spleen lysate
    Lane 10 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 11 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 138 kDa
    Observed band size: 125,155,186,75 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).

    The 75 kDa band represents one of the isoforms annotated in UniProt.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm and membrane staining on myenteric nerve plexus, and negative on epithelium cells and smooth muscle cells of mouse colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

References

ab184377 has not yet been referenced specifically in any publications.

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