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    neurofibromin-antibody-ab128054.pdf

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Neuroscience Neurotransmission Intracellular Signaling Cytoskeletal
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Anti-Neurofibromin antibody (ab128054)

  • Datasheet
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Western blot - Anti-Neurofibromin antibody (ab128054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofibromin antibody (ab128054)

Key features and details

  • Rabbit polyclonal to Neurofibromin
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Neurofibromin antibody
    See all Neurofibromin primary antibodies
  • Description

    Rabbit polyclonal to Neurofibromin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to an internal region of Human Neurofibromin.

  • Positive control

    • HepG2 cell extract, Human spleen and Human tonsil tissues, HepG2 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Cytoskeletal
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab128054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 319 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
  • Involvement in disease

    Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
    Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
    Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
    Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
    Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
    Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities

    Contains 1 CRAL-TRIO domain.
    Contains 1 Ras-GAP domain.
  • Target information above from: UniProt accession P21359 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4763 Human
    • Entrez Gene: 18015 Mouse
    • Entrez Gene: 24592 Rat
    • Omim: 613113 Human
    • SwissProt: P21359 Human
    • SwissProt: Q04690 Mouse
    • SwissProt: P97526 Rat
    • Unigene: 113577 Human
    • Unigene: 255596 Mouse
    • Unigene: 10686 Rat
    see all
  • Alternative names

    • DKFZp686J1293 antibody
    • FLJ21220 antibody
    • Neurofibromatosis Noonan syndrome antibody
    • Neurofibromatosis related protein NF 1 antibody
    • Neurofibromatosis related protein NF1 antibody
    • neurofibromatosis type I antibody
    • Neurofibromatosis-related protein NF-1 antibody
    • Neurofibromin 1 antibody
    • Neurofibromin truncated antibody
    • Neurofibromin1 antibody
    • NF 1 antibody
    • NF antibody
    • NF1 antibody
    • NF1_HUMAN antibody
    • NFNS antibody
    • Type 1 Neurofibromatosis antibody
    • von Recklinghausen disease neurofibromin antibody
    • von Recklinghausen disease related protein VRNF antibody
    • VRNF antibody
    • WATS antibody
    • Watson disease related protein WSS antibody
    • Watson syndrome antibody
    • WSS antibody
    see all

Images

  • Western blot - Anti-Neurofibromin antibody (ab128054)
    Western blot - Anti-Neurofibromin antibody (ab128054)
    All lanes : Anti-Neurofibromin antibody (ab128054) at 1/500 dilution

    Lane 1 : HepG2 cells extract
    Lane 2 : HepG2 cells extract with synthesized peptide

    Predicted band size: 319 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
    ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)
    ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofibromin antibody (ab128054)
    Immunocytochemistry/ Immunofluorescence - Anti-Neurofibromin antibody (ab128054)
    ab128054, at a dilution of 1/100, staining Neurofibromin in HepG2 cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab128054? Please let us know so that we can cite the reference in this datasheet.

    ab128054 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hello,

    I'm about to order the antibodiesab128054 andab30325 for neurofibromin. Before I place the order I'd like to ask a question regarding the immunogen for ab128054. Is it possible to say approx where in the protein the immunogen is? Is it closer to the C-terminus than to the N-? If it is impossible to let out the specific amino acids, maybe it is possible to say in which quartile it is located?

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for your question.

    I am sorry to confirm that regrettablythe lab that provides this antibody considers the immunogenic peptide information to be proprietary in this case. The only information that we have is that the peptide is from an internal region of the protein. So, not at the N-terminal or the C-terminal.

    We aim to provide as much information as possible to customers, and I am sorry there are no more specific details to send on this occasion. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on May 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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