Anti-Neurofibromin antibody (ab128054)
Key features and details
- Rabbit polyclonal to Neurofibromin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Neurofibromin antibody
See all Neurofibromin primary antibodies -
Description
Rabbit polyclonal to Neurofibromin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide, corresponding to an internal region of Human Neurofibromin.
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Positive control
- HepG2 cell extract, Human spleen and Human tonsil tissues, HepG2 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
(without Mg2+, Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab128054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Predicted molecular weight: 319 kDa. | |
IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/500. |
Target
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Function
Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. -
Involvement in disease
Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Contains 1 CRAL-TRIO domain.
Contains 1 Ras-GAP domain. - Information by UniProt
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Database links
- Entrez Gene: 4763 Human
- Entrez Gene: 18015 Mouse
- Entrez Gene: 24592 Rat
- Omim: 613113 Human
- SwissProt: P21359 Human
- SwissProt: Q04690 Mouse
- SwissProt: P97526 Rat
- Unigene: 113577 Human
see all -
Alternative names
- DKFZp686J1293 antibody
- FLJ21220 antibody
- Neurofibromatosis Noonan syndrome antibody
see all
Images
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All lanes : Anti-Neurofibromin antibody (ab128054) at 1/500 dilution
Lane 1 : HepG2 cells extract
Lane 2 : HepG2 cells extract with synthesized peptide
Predicted band size: 319 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human spleen tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (ab128054)ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
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ab128054, at a dilution of 1/100, staining Neurofibromin in HepG2 cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
References (0)
ab128054 has not yet been referenced specifically in any publications.