Anti-Neurofilament antibody (ab204893)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Neurofilament antibody
See all Neurofilament primary antibodies -
Description
Rabbit polyclonal to Neurofilament -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human Neurofilament aa 410-473.
Sequence:ECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEE QTEETQVTEEVTEE
Database link: P12036 -
Positive control
- Human cerebral cortex tissue; SiHa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab204893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Use PFA/Triton X-100 Fixation/Permeabilization. |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 4741 Human
- Entrez Gene: 4744 Human
- Entrez Gene: 4747 Human
- Entrez Gene: 18039 Mouse
- Entrez Gene: 18040 Mouse
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- Entrez Gene: 24588 Rat
see all -
Alternative names
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- 2 kDa neurofilament protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament antibody (ab204893)Immunofluorescent analysis of SiHa cells (PFA-fixed/Triton X-100 permeabilized) labeling Neurofilament with ab204893 at 4 µg/ml (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (ab204893)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Neurofilament with ab204893 at 1/200 dilution.
Datasheets and documents
References
This product has been referenced in:
- Ahn JH et al. Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid. Nature 572:62-66 (2019). Read more (PubMed: 31341278) »
