Product nameAnti-Neurofilament antibody [RNF406]
See all Neurofilament primary antibodies
DescriptionMouse monoclonal [RNF406] to Neurofilament
Specificityab134306 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Hamster, Cow, Xenopus laevis, Monkey
Neurofilament preparation of calf brain tissue.
- Human brain cortex tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab134306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 102 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-Fr||1/50 - 1/100.|
|Flow Cyt||1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- 2 kDa neurofilament protein antibody
All lanes : Anti-Neurofilament antibody [RNF406] (ab134306) at 1/100 dilution
Lane 1 : Wild-type HEK293 whole cell lysate
Lane 2 : Neurofilament knockout HEK293 whole cell lysate
Lane 3 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 102 kDa
Observed band size: 140-160 kDa why is the actual band size different from the predicted?
Lanes 1 - 3: Merged signal (red and green). Green - ab134306 observed at 140-160 kDa kDa. Red - loading control, ab52866, observed at 50 kDa.
ab134306 was shown to specifically react with Neurofilament in wild-type HEK293 cells as signal was lost in Neurofilament knockout cells. Wild-type and Neurofilament knockout samples were subjected to SDS-PAGE. ab134306 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labelling 140 kD + 160 kD Neurofilament with ab134306 at 10 µg/ml, using a biotinylated horse anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
This product has been referenced in:
- Rolland-Fourcade C et al. Epithelial expression and function of trypsin-3 in irritable bowel syndrome. Gut 66:1767-1778 (2017). Read more (PubMed: 28096305) »
- Bryukhovetskiy IS et al. Hematopoietic stem cells as a tool for the treatment of glioblastoma multiforme. Mol Med Rep 14:4511-4520 (2016). Human . Read more (PubMed: 27748891) »