Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-FoFr, WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgY
Product nameAnti-Neurofilament heavy polypeptide antibody
See all Neurofilament heavy polypeptide primary antibodies
DescriptionChicken polyclonal to Neurofilament heavy polypeptide
Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.
- Dissociated cell culture prepared from an adult rat brain.
Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
Purification notesImmune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer, and then filter-sterilized.
Our Abpromise guarantee covers the use of ab134459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/2000 - 1/5000. Use 2% paraformaldehyde-fixed tissues.|
|WB||1/5000 - 1/10000. Predicted molecular weight: 112 kDa.|
|ICC/IF||1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.|
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
ab134459 has been referenced in 2 publications.
- Szobota S et al. BDNF, NT-3 and Trk receptor agonist monoclonal antibodies promote neuron survival, neurite extension, and synapse restoration in rat cochlea ex vivo models relevant for hidden hearing loss. PLoS One 14:e0224022 (2019). PubMed: 31671109
- Park SH et al. Adeno-associated virus serotype rh10 is a useful gene transfer vector for sensory nerves that innervate bone in immunodeficient mice. Sci Rep 7:17428 (2017). PubMed: 29233995