Key features and details
- Rabbit polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Neurofilament heavy polypeptide antibody
See all Neurofilament heavy polypeptide primary antibodies
DescriptionRabbit polyclonal to Neurofilament heavy polypeptide
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- IHC-P: Mouse brain, rat brain and spinal cord tissues.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe please use dilutions of 1/50 - 1/200.
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse brain tissue, labeling Neurofilament using ab203039 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat spinal cord tissue, labeling Neurofilament using ab203039 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling Neurofilament using ab203039 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab203039 has not yet been referenced specifically in any publications.