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  1. Link

    neurofilament-heavy-polypeptide-antibody-ab4680.pdf

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Anti-Neurofilament heavy polypeptide antibody (ab4680)

  • Datasheet
  • SDS
Reviews (12)Q&A (1)References (96)

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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

Key features and details

  • Chicken polyclonal to Neurofilament heavy polypeptide
  • Suitable for: ICC, IHC-FrFl, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody
    See all Neurofilament heavy polypeptide primary antibodies
  • Description

    Chicken polyclonal to Neurofilament heavy polypeptide
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC, IHC-FrFl, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development

Associated products

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (1)
Use at an assay dependent concentration.
IHC-FrFl (1)
Use at an assay dependent concentration.
WB
1/1e+006. Predicted molecular weight: 200-220 kDa.
Notes
ICC
Use at an assay dependent concentration.
IHC-FrFl
Use at an assay dependent concentration.
WB
1/1e+006. Predicted molecular weight: 200-220 kDa.

Target

  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in disease

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 528842 Cow
    • Entrez Gene: 4744 Human
    • Entrez Gene: 380684 Mouse
    • Entrez Gene: 24587 Rat
    • Omim: 162230 Human
    • SwissProt: P12036 Human
    • SwissProt: P19246 Mouse
    • SwissProt: P16884 Rat
    • Unigene: 198760 Human
    • Unigene: 298283 Mouse
    • Unigene: 108194 Rat
    see all
  • Alternative names

    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Images

  • Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

    Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.

  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution

    Lane 1 : Rat spinal cord lysate
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Cow spinal cord lysate

    Predicted band size: 200-220 kDa

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

    Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048

    ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.

Protocols

  • Immunostaining of Cells in Tissue Culture

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (96)

Publishing research using ab4680? Please let us know so that we can cite the reference in this datasheet.

ab4680 has been referenced in 96 publications.

  • Hosoya M  et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
  • Ewan EE  et al. Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism. Sci Rep 11:374 (2021). PubMed: 33431991
  • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
  • Triplet EM  et al. The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiol Dis 152:105294 (2021). PubMed: 33549720
  • Andreassi C  et al. Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep 34:108778 (2021). PubMed: 33626357
View all Publications for this product

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Read More

Abcam user community

Verified customer

Submitted Oct 09 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - 1%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted Feb 18 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
None
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted Mar 22 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Permeabilization
No
Specification
spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Read More

MR. Tamas Bellak

Verified customer

Submitted Mar 19 2018

Immunocytochemistry abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Differentiating Neuronal cells)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Differentiating Neuronal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 17 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Pig Cell (Dorsal root ganglion neuron)
Specification
Dorsal root ganglion neuron
Permeabilization
Yes - Triton X
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 20 2015

Immunohistochemistry (Frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (dorsal root ganglian)
Specification
dorsal root ganglian
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 16 2015

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
mix BSA 1% and Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (BRAIN)
Specification
BRAIN
Permeabilization
Yes - Triton 2%
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted Apr 30 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA1% + Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Aplysia Tissue sections (buccal ganglia)
Specification
buccal ganglia
Permeabilization
Yes - triton 2%
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted Sep 30 2013

IHC - Wholemount abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (never)
Specification
never
Read More

DR. Xinpeng Dun

Verified customer

Submitted Jul 05 2012

1-10 of 13 Abreviews or Q&A

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