• Product name
    Anti-Neurofilament heavy polypeptide antibody
    See all Neurofilament heavy polypeptide primary antibodies
  • Description
    Chicken polyclonal to Neurofilament heavy polypeptide
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, IHC-FoFr, IHC - Wholemount, ICC/IF, IHC-FrFl, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Aplysia
  • Immunogen

    Full length native protein (cow intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell).

  • Positive control
    • IHC-FrFl: Rat cerebellum tissue. WB: Mouse, rat and cow spinal cord lysate. IHC-PerFo: Aplysia buccal ganglia. ICC/IF: Mixed neuron/glial cultures. SH-SY5Y cells. Rat neurons. Murine embryoid bodies. IHC-Fr: Rat skin tissue.



Our Abpromise guarantee covers the use of ab4680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10000.
IHC-FoFr 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19944698
IHC-FrFl Use at an assay dependent concentration.
WB 1/1e+006. Predicted molecular weight: 200-220 kDa.


  • Function
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in disease
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all


  • Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

    Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.

  • All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution

    Lane 1 : Rat spinal cord lysate
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Cow spinal cord lysate

    Predicted band size: 200-220 kDa

  • Immunohistochemical staining of Neurofilament heavy polypeptide in aplysia buccal ganglia with ab4680 at 1/1000. The sample was permeabilized with 2% Triton X100 and blocked with 1%BSA/5% goat serum for 2 hours at room temperature, before being incubated with the primary antibody for 1 hour at room temperature in 5% goat serum. ab96949 (goat anti-chicken IgY H&L DyLight® 594) was used as the secondary antibody at 1/200.

    See Abreview

  • Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

  • Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
  • Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

  • ab4680 at 1/10000 dilution staining Neurofilament heavy polypeptide in rat skin tissue by immunohistochemistry (frozen sections). Sections were fixed using Bouin's Fixative and permeabilized in 0.1% Triton X-100 prior to blocking in 10% serum for 1 hour at 24°C and then incubated with ab4680 for 24 hours at 4°C. A donkey polyclonal to chicken IgY, diluted 1/100 was used as the secondary antibody.

    See Abreview

  • ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.


This product has been referenced in:
  • Lu Y  et al. Fibroblast growth factor 21 facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagic cell death. J Cell Mol Med 23:497-511 (2019). Read more (PubMed: 30450828) »
  • Dun XP  et al. Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge. Cell Rep 26:1458-1472.e4 (2019). Read more (PubMed: 30726731) »
See all 45 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry. A positive control for immunocytochemistry would be mixed neuron/glial culture or frozen sections of brain or spinal cord. In either case rat, mouse or any other mammal should be fine. The antibody stains axons very strongly and specifically at dilutions down to 1:100,000 using fluorescent secondary antibodies. For a positive control for western blots a urea extract of spinal cord works well. We homogenize 10mg/ml wet weight of fresh mammalian spinal cord in 6M urea in PBS plus 0.1% beta-mercaptoethanol. We pellet out insoluble material in an airfuge, TL100, TL130 or similar and run the supernatant on SDS-PAGE gels at 5 micrograms wet weight per lane. The antibody stains a major band at 200kDa to 220kDa band, depending on the species, corresponding to the axonal form of NF-H. (for whatever reason, larger animals tend to have NF-H with a larger apparent molecular weight, with mouse and rat about 200kDa, but pig, cow and human all at about 220kDa). Again the antibody has an extremely high titer so it may be necessary to dilute to 1:1,000,000 or more if chemiluminescence is being used.

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