Immunohistochemistry (Frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (scatic nerve)
Specification
scatic nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% TX-100 30min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Other product details

Dilution
1/10000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti chicken 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/500

Abcam user community

Verified customer

Submitted May 29 2012

Sign up