Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-200 kD Neurofilament Heavy antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
mix BSA 1% and Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (BRAIN)
Specification
BRAIN
Permeabilization
Yes - Triton 2%
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: goat serum 5%
Dilution
1/10000

Secondary antibody

Dilution
1/250

Mrs. Francoise Geffroy

Verified customer

Submitted Apr 30 2014

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