Key features and details
- Rabbit polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-Neurofilament heavy polypeptide antibody
See all Neurofilament heavy polypeptide primary antibodies
DescriptionRabbit polyclonal to Neurofilament heavy polypeptide
SpecificityThe antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
Tested applicationsSuitable for: IHC-FrFl, WBmore details
Species reactivityReacts with: Mouse, Rat
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
- Information by UniProt
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Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
ab8135 has been referenced in 71 publications.
- Serrano GE et al. Cardiac sympathetic denervation and synucleinopathy in Alzheimer's disease with brain Lewy body disease. Brain Commun 2:fcaa004 (2020). PubMed: 32064463
- You H et al. Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Sci Adv 6:eaax6040 (2020). PubMed: 32076639
- Jensen BK et al. Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment. EMBO Mol Med 12:e10722 (2020). PubMed: 32347002
- Hu X et al. Thermosensitive heparin-poloxamer hydrogel encapsulated bFGF and NGF to treat spinal cord injury. J Cell Mol Med 24:8166-8178 (2020). PubMed: 32515141
- Das S et al. Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss. Commun Biol 3:330 (2020). PubMed: 32587337
Customer reviews and Q&As
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