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  1. Link

    neurofilament-heavy-polypeptide-antibody-ab8135.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other
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Anti-Neurofilament heavy polypeptide antibody (ab8135)

  • Datasheet
  • SDS
Reviews (25)Q&A (3)References (94)

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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
  • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)

Key features and details

  • Rabbit polyclonal to Neurofilament heavy polypeptide
  • Suitable for: IHC-FrFl, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody
    See all Neurofilament heavy polypeptide primary antibodies
  • Description

    Rabbit polyclonal to Neurofilament heavy polypeptide
  • Host species

    Rabbit
  • Specificity

    The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
  • Tested applications

    Suitable for: IHC-FrFl, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
    Database link: F1MSQ6

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.03% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl (1)
1/5000.
WB (2)
1/10000.
Notes
IHC-FrFl
1/5000.
WB
1/10000.

Target

  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in disease

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 380684 Mouse
    • Entrez Gene: 24587 Rat
    • SwissProt: P19246 Mouse
    • SwissProt: P16884 Rat
    • Unigene: 298283 Mouse
    • Unigene: 108194 Rat
    • Alternative names

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    Images

    • Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)

      Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

      Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

    • Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)
      All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution

      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat spinal cord tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : Mouse spinal cord tissue lysate

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (94)

    Publishing research using ab8135? Please let us know so that we can cite the reference in this datasheet.

    ab8135 has been referenced in 94 publications.

    • Li H  et al. Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction. Transl Androl Urol 10:258-271 (2021). PubMed: 33532315
    • Manousi A  et al. Identification of novel myelin repair drugs by modulation of oligodendroglial differentiation competence. EBioMedicine 65:103276 (2021). PubMed: 33714029
    • Liu H  et al. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-?B signaling. Theranostics 11:4187-4206 (2021). PubMed: 33754056
    • Feng Y  et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
    • Mughrabi IT  et al. Development and characterization of a chronic implant mouse model for vagus nerve stimulation. Elife 10:N/A (2021). PubMed: 33821789
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 28 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Sciatic nerve)
    Specification
    Sciatic nerve
    Read More

    MS. Lolita Singh

    Verified customer

    Submitted Nov 20 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    MR. Victor Bernal

    Verified customer

    Submitted Aug 26 2020

    Immunohistochemistry free floating abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Read More

    Mr. Daniel Lustberg

    Verified customer

    Submitted Aug 01 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Differentiated iPS cells)
    Permeabilization
    Yes - 0.3% Triton + 0.1% BSA
    Specification
    Differentiated iPS cells
    Blocking step
    0.1% BSA/10%FCS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 03 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate ph 6
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Herr Dr. Markus Kipp

    Verified customer

    Submitted Sep 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Skin\whiskers)
    Permeabilization
    No
    Specification
    Skin\whiskers
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    MR. Elena Ezhkova

    Verified customer

    Submitted Jan 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Nerve)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: ProK
    Permeabilization
    No
    Specification
    Nerve
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 11 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (engineered nervous tissue)
    Permeabilization
    No
    Specification
    engineered nervous tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 22 2014

    1-10 of 28 Abreviews or Q&A

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