Anti-Neurofilament heavy polypeptide antibody (ab8135)
Key features and details
- Rabbit polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody
See all Neurofilament heavy polypeptide primary antibodies -
Description
Rabbit polyclonal to Neurofilament heavy polypeptide -
Host species
Rabbit -
Specificity
The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa). -
Tested applications
Suitable for: IHC-FrFl, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
Database link: F1MSQ6 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl | (1) |
1/5000.
|
| WB | (2) |
1/10000.
|
| Notes |
|---|
|
IHC-FrFl
1/5000. |
|
WB
1/10000. |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
Involvement in disease
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
- Unigene: 298283 Mouse
- Unigene: 108194 Rat
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Alternative names
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
Images
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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
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Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (94)
ab8135 has been referenced in 94 publications.
- Li H et al. Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction. Transl Androl Urol 10:258-271 (2021). PubMed: 33532315
- Manousi A et al. Identification of novel myelin repair drugs by modulation of oligodendroglial differentiation competence. EBioMedicine 65:103276 (2021). PubMed: 33714029
- Liu H et al. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-?B signaling. Theranostics 11:4187-4206 (2021). PubMed: 33754056
- Feng Y et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
- Mughrabi IT et al. Development and characterization of a chronic implant mouse model for vagus nerve stimulation. Elife 10:N/A (2021). PubMed: 33821789