Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

Reviews (4)Q&A (2)References (14)

Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody [NF-01]
    See all Neurofilament heavy polypeptide primary antibodies

  • Description

    Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide

  • Host species

    Mouse

  • Specificity

    This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC, IHC-P, WB, IHC - Wholemount, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig
    Predicted to work with: a wide range of other species, Mammals

  • Immunogen

    A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

Properties

Applications

Our Abpromise guarantee covers the use of ab7795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

  • Involvement in disease

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.

  • Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
    Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

    Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488  (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

Publishing research using ab7795? Please let us know so that we can cite the reference in this datasheet.

ab7795 has been referenced in 14 publications.

  • Komura H  et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). PubMed: 30827871
  • Galino J  et al. RalGTPases contribute to Schwann cell repair after nerve injury via regulation of process formation. J Cell Biol 218:2370-2387 (2019). PubMed: 31201266
  • Chen X  et al. Functional Multichannel Poly(Propylene Fumarate)-Collagen Scaffold with Collagen-Binding Neurotrophic Factor 3 Promotes Neural Regeneration After Transected Spinal Cord Injury. Adv Healthc Mater 7:e1800315 (2018). PubMed: 29920990
  • Tebaldi T  et al. HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA. Mol Cell 71:256-270.e10 (2018). PubMed: 30029004
  • Pujol-Calderón F  et al. Neurofilament changes in serum and cerebrospinal fluid after acute ischemic stroke. Neurosci Lett 698:58-63 (2018). PubMed: 30599262
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Recurrent Laryngeal Nerve)
Permeabilization
No
Specification
Recurrent Laryngeal Nerve
Fixative
Frozen in Isopentane and Liquid Nitrogen
Read More

Stephen Cahalan

Verified customer

Submitted May 10 2017

Immunocytochemistry abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

 Average
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (mESC)
Permeabilization
Yes - tritonx100
Specification
mESC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Kyungjoo Seong

Verified customer

Submitted Nov 02 2016

IHC - Wholemount abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

 Good
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Rat Tissue (Dorsal root ganglion)
Specification
Dorsal root ganglion
Read More

Dr. Shlomo Tsuriel

Verified customer

Submitted Feb 06 2014

Question

To whom correspond,

Dear Madame/Sir,

we used frequently the antibody mouse SMI-32 to bind to Non-phosphorylated Neurofilament Heavy Chain (old reference in Abcam: ab-73273-250, 330E per vial). We have noticed you do not sold this refecence anymore. Could you suggest another antibody reference to replace that one?

Thank you very much in advance.

Kind regards,
--

Read More
Answer

Thank you for your inquiry.

I can confirm that we do not have ab73273 in our catalog anymore.

I can suggest ab7795 as alternative. It also recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

https://www.abcam.com/index.html?datasheet=7795 (or use the following: https://www.abcam.com/index.html?datasheet=7795).

I hope this information is helpful and wish you good luck with your research.

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Differentiated embryonal carcinoma cells)
Specification
Differentiated embryonal carcinoma cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Mar 18 2009

Question

We intend to order this antibody providing it works in mouse tissues. Can you guarantee this? if not, can you please suggest an alternative? Thank you very much.

Read More
Answer

Please refer the paper on the datasheet (Histochemistry 100, 495,1993) which features the use of this monoclonal antibody. "In similar preparations (the authors refer to pellet of brain proteins after cold depolymerization, described elsewhere in the text) isolated from mouse and rat brains, the antibody also reacted with the protein at the NF-H position." The paper follows expression of phosphorylated NF-H in human developing dorsal root ganglia and spinal cord and makes no other note about reactivity of ab7795 with mouse tissue sections. To summarise, we are pretty sure that the antibody cross reacts with mouse.

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