Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

Reviews (5)Q&A (2)References (18)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
  • Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

Key features and details

  • Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody [NF-01]
    See all Neurofilament heavy polypeptide primary antibodies

  • Description

    Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide

  • Host species

    Mouse

  • Specificity

    This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species, Mammals

  • Immunogen

    A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

  • Involvement in disease

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt

  • Database links

    • Alternative names

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.

    • Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
      Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

      Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488  (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References (18)

    Publishing research using ab7795? Please let us know so that we can cite the reference in this datasheet.

    ab7795 has been referenced in 18 publications.

    • Lukácová N  et al. Is Innervation of the Neuromuscular Junction at the Diaphragm Modulated by sGC/cGMP Signaling? Front Physiol 11:700 (2020). PubMed: 32655417
    • Schoonover KE  et al. Pathology of white matter integrity in three major white matter fasciculi: A post-mortem study of schizophrenia and treatment status. Br J Pharmacol 176:1143-1155 (2019). PubMed: 30735241
    • Komura H  et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). PubMed: 30827871
    • Galino J  et al. RalGTPases contribute to Schwann cell repair after nerve injury via regulation of process formation. J Cell Biol 218:2370-2387 (2019). PubMed: 31201266
    • Marton RM  et al. Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures. Nat Neurosci 22:484-491 (2019). PubMed: 30692691
    View all Publications for this product

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High pH
    Specification
    Brain
    Blocking step
    (agent) for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Mark Butters

    Verified customer

    Submitted Feb 02 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Horse Tissue sections (Recurrent Laryngeal Nerve)
    Permeabilization
    No
    Specification
    Recurrent Laryngeal Nerve
    Fixative
    Frozen in Isopentane and Liquid Nitrogen
    Read More

    Stephen Cahalan

    Verified customer

    Submitted May 10 2017

    Immunocytochemistry abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (mESC)
    Permeabilization
    Yes - tritonx100
    Specification
    mESC
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Kyungjoo Seong

    Verified customer

    Submitted Nov 02 2016

    IHC - Wholemount abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

     Good
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Dorsal root ganglion)
    Specification
    Dorsal root ganglion
    Read More

    Dr. Shlomo Tsuriel

    Verified customer

    Submitted Feb 06 2014

    Question

    To whom correspond,

    Dear Madame/Sir,

    we used frequently the antibody mouse SMI-32 to bind to Non-phosphorylated Neurofilament Heavy Chain (old reference in Abcam: ab-73273-250, 330E per vial). We have noticed you do not sold this refecence anymore. Could you suggest another antibody reference to replace that one?

    Thank you very much in advance.

    Kind regards,
    --

    Read More
    Answer

    Thank you for your inquiry.

    I can confirm that we do not have ab73273 in our catalog anymore.

    I can suggest ab7795 as alternative. It also recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

    https://www.abcam.com/Neurofilament-heavy-polypeptide-antibody-NF-01-ab7795.html (or use the following: https://www.abcam.com/Neurofilament-heavy-polypeptide-antibody-NF-01-ab7795.html).

    I hope this information is helpful and wish you good luck with your research.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Differentiated embryonal carcinoma cells)
    Specification
    Differentiated embryonal carcinoma cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2009

    Question

    We intend to order this antibody providing it works in mouse tissues. Can you guarantee this? if not, can you please suggest an alternative? Thank you very much.

    Read More
    Answer

    Please refer the paper on the datasheet (Histochemistry 100, 495,1993) which features the use of this monoclonal antibody. "In similar preparations (the authors refer to pellet of brain proteins after cold depolymerization, described elsewhere in the text) isolated from mouse and rat brains, the antibody also reacted with the protein at the NF-H position." The paper follows expression of phosphorylated NF-H in human developing dorsal root ganglia and spinal cord and makes no other note about reactivity of ab7795 with mouse tissue sections. To summarise, we are pretty sure that the antibody cross reacts with mouse.

    Read More

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