Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody [NF-01]
See all Neurofilament heavy polypeptide primary antibodies -
Description
Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide -
Host species
Mouse -
Specificity
This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC, IHC-P, WB, IHC - Wholemount, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig
Predicted to work with: a wide range of other species, Mammals
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Immunogen
A pellet of pig brain cold-stable proteins after depolymerization of microtubules.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
>95 % pure (by PAGE) -
Clonality
Monoclonal -
Clone number
NF-01 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab7795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000. | |
| IHC - Wholemount | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
Involvement in disease
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 528842 Cow
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 100156492 Pig
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
see all -
Alternative names
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)](https://www.abcam.com/ps/products/7/ab7795/Images/ab7795-265224-anti-neurofilament-heavy-polypeptide-antibody-nf-01-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.
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![Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)](https://www.abcam.com/ps/products/7/ab7795/Images/ab7795-13258-anti-neurofilament-heavy-polypeptide-antibody-nf-01-flow-cytometry.jpg)
Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References
This product has been referenced in:
- Komura H et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). Read more (PubMed: 30827871) »
- Galino J et al. RalGTPases contribute to Schwann cell repair after nerve injury via regulation of process formation. J Cell Biol 218:2370-2387 (2019). Read more (PubMed: 31201266) »
