Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
Key features and details
- Mouse monoclonal [NF421] to Neurofilament heavy polypeptide
- Suitable for: WB, ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody [NF421]
See all Neurofilament heavy polypeptide primary antibodies -
Description
Mouse monoclonal [NF421] to Neurofilament heavy polypeptide -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Pig -
Immunogen
Recombinant full length protein corresponding to Human Neurofilament heavy polypeptide aa 1-1026.
Database link: 4744 -
Positive control
- IHC-P: Human and rat cerebellum tissues. ICC: HEK-293 cells. WB: Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by Protein A/G. -
Clonality
Monoclonal -
Clone number
NF421 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 112 kDa.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 112 kDa. |
ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
Involvement in disease
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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Database links
- Entrez Gene: 417020 Chicken
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 100156492 Pig
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
see all -
Alternative names
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.
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Immunocytochemistry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml (green). Membrane stained with Phalloidin (red).
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Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374) at 1 µg/ml + Human brain tissue lysate
Predicted band size: 112 kDaWestern blot analysis of human brain tissue lysate labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab187374 has been referenced in 2 publications.
- Shenton FC et al. Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart. J Anat 238:36-52 (2021). PubMed: 32783212
- Bonaventura G et al. Ag-NPs induce apoptosis, mitochondrial damages and MT3/OSGIN2 expression changes in an in vitro model of human dental-pulp-stem-cells-derived neurons. Neurotoxicology 67:84-93 (2018). PubMed: 29698629