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    neurogenin-1neurog1-antibody-ab66498.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-Neurogenin 1/NEUROG1 antibody (ab66498)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)

Key features and details

  • Rabbit polyclonal to Neurogenin 1/NEUROG1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Neurogenin 1/NEUROG1 antibody
    See all Neurogenin 1/NEUROG1 primary antibodies
  • Description

    Rabbit polyclonal to Neurogenin 1/NEUROG1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Neurogenin 1/NEUROG1 (C terminal).

  • General notes

     This product was previously labelled as Neurogenin 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Neural Stem Cells
    • Intracellular

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
All applications
Mouse
Application Abreviews Notes
IHC-P
1/1000.
ICC/IF
1/1000.
Notes
IHC-P
1/1000.
ICC/IF
1/1000.

Target

  • Function

    Appears to mediate neuronal differentiation.
  • Tissue specificity

    Expression restricted to the embryonic nervous system.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q92886 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4762 Human
    • Entrez Gene: 18014 Mouse
    • Omim: 601726 Human
    • SwissProt: Q92886 Human
    • SwissProt: P70660 Mouse
    • Unigene: 248149 Human
    • Unigene: 266665 Mouse
    • Alternative names

      • AKA antibody
      • Ath4c antibody
      • bHLHa6 antibody
      • Class A basic helix-loop-helix protein 6 antibody
      • Helix loop helix protein mATH 4C antibody
      • MATH4C antibody
      • NeuroD3 antibody
      • Neurog 1 antibody
      • Neurog1 antibody
      • Neurogenic basic helix loop helix protein antibody
      • Neurogenic basic-helix-loop-helix protein antibody
      • Neurogenic differentiation 3 antibody
      • Neurogenic differentiation factor 3 antibody
      • Neurogenin-1 antibody
      • Neurogenin1 antibody
      • Ngn antibody
      • NGN-1 antibody
      • Ngn1 antibody
      • NGN1_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
      Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
      ICC/IF image of ab66498 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66498, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
      Ab66498 staining human normal liver. Staining is localised to the nucleus.
      Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab66498? Please let us know so that we can cite the reference in this datasheet.

    ab66498 has been referenced in 3 publications.

    • Han S  et al. A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis. Development 145:N/A (2018). PubMed: 30201687
    • Zhang X  et al. Impact of siRNA targeting of ß-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Mol Med Rep 14:3595-601 (2016). WB ; Rat . PubMed: 27573468
    • Blanchard JW  et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). ICC ; Mouse . PubMed: 25420069

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Neurogenin 1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E11.5 mouse embryos transverse section)
    Specification
    E11.5 mouse embryos transverse section
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
    Blocking step
    NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2013

    Question

    No specific staining of human neuroprogenitor cells.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2011

    Answer

    Thank you for your call today and for letting us know about the trouble with ab66498. As we discussed, I am sending a free of charge vial of ab89461 on the order ***, which I expect to arrive Friday of next week or shortly after. Please keep me updated of the results with this replacement, and let me know if you need anything else.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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