Anti-Neurogenin 1/NEUROG1 antibody (ab66498)
Key features and details
- Rabbit polyclonal to Neurogenin 1/NEUROG1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Neurogenin 1/NEUROG1 antibody
See all Neurogenin 1/NEUROG1 primary antibodies -
Description
Rabbit polyclonal to Neurogenin 1/NEUROG1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
Immunogen
Synthetic peptide corresponding to Human Neurogenin 1/NEUROG1 (C terminal).
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General notes
This product was previously labelled as Neurogenin 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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All applications |
Mouse
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Application | Abreviews | Notes |
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IHC-P |
1/1000.
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ICC/IF |
1/1000.
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Notes |
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IHC-P
1/1000. |
ICC/IF
1/1000. |
Target
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Function
Appears to mediate neuronal differentiation. -
Tissue specificity
Expression restricted to the embryonic nervous system. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4762 Human
- Entrez Gene: 18014 Mouse
- Omim: 601726 Human
- SwissProt: Q92886 Human
- SwissProt: P70660 Mouse
- Unigene: 248149 Human
- Unigene: 266665 Mouse
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Alternative names
- AKA antibody
- Ath4c antibody
- bHLHa6 antibody
see all
Images
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ICC/IF image of ab66498 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66498, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (ab66498)Ab66498 staining human normal liver. Staining is localised to the nucleus.
Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab66498 has been referenced in 3 publications.
- Han S et al. A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis. Development 145:N/A (2018). PubMed: 30201687
- Zhang X et al. Impact of siRNA targeting of ß-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Mol Med Rep 14:3595-601 (2016). WB ; Rat . PubMed: 27573468
- Blanchard JW et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). ICC ; Mouse . PubMed: 25420069