Overview

  • Product name

    Anti-Neurogenin 2/NGN2 antibody
    See all Neurogenin 2/NGN2 primary antibodies
  • Description

    Rabbit polyclonal to Neurogenin 2/NGN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Neurogenin 2/NGN2 aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P70447

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse E10 Embryonic Brain; Rat E14 Embryonic Brain; Rat E14 Embryonic Spinal Cord; Rat E16 Embryonic Brain; Rat E16 Embryonic Spinal Cord. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
  • General notes

     This product was previously labelled as Neurogenin 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • Function

    Involved in neurogenesis.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atoh 4 antibody
    • Atoh4 antibody
    • bHLHa8 antibody
    • Class A basic helix-loop-helix protein 8 antibody
    • Math 4A antibody
    • Math4A antibody
    • MGC antibody
    • MGC46562 antibody
    • NEUROG 2 antibody
    • NEUROG2 antibody
    • Neurogenin-2 antibody
    • Neurogenin2 antibody
    • NGN 2 antibody
    • NGN-2 antibody
    • NGN2 antibody
    • NGN2_HUMAN antibody
    • Protein atonal homolog 4 antibody
    see all

Images

  • ICC/IF image of ab154293 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Triton for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154293 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Neurogenin 2/NGN2 antibody (ab154293) at 1 µg/ml (Milk blocking 5%)

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E14 Rat Embryo Brain Tissue Lysate
    Lane 3 : E14 Rat Embryo Spinal Cord Tissue Lysate
    Lane 4 : E16 Rat Embryo Brain Tissue Lysate
    Lane 5 : E16 Rat Embryo Spinal Cord Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab154293 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • All lanes : Anti-Neurogenin 2/NGN2 antibody (ab154293) at 1 µg/ml

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E14 Rat Embryo Brain Tissue Lysate
    Lane 3 : E14 Rat Embryo Spinal Cord Tissue Lysate
    Lane 4 : E16 Rat Embryo Brain Tissue Lysate
    Lane 5 : E16 Rat Embryo Spinal Cord Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa
    Additional bands at: 100 kDa (possible non-specific binding), 150 kDa (possible non-specific binding), 48 kDa (possible non-specific binding)


    Exposure time: 90 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154293 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab154293 has not yet been referenced specifically in any publications.

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