Recombinant
RabMAb

Recombinant Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

Reviews (1)Q&A (1)

Overview

  • Product name

    Anti-Neurogenin 2/NGN2 antibody [EPR4177]
    See all Neurogenin 2/NGN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Neurogenin 2/NGN2 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H2A3
    (Peptide available as ab187850)

  • Positive control

    • WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.

  • General notes

     

     

     This product was previously labelled as Neurogenin 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    Images

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)

      Lane 1 : PC-12 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 29 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 29 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    ab109236 has not yet been referenced specifically in any publications.

    Publishing research using ab109236? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Neurogenin 2/NGN2 antibody [EPR4177]

     Poor
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Retina)
    Permeabilization
    Yes - 0.3% triton
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 21 2014

    Question

    Customer kindly contacted us that ab109236 has not been giving a strong band in western blot.
    Application: Western blot
    Primary: 1/1000
    Block: milk
    Sample: mouse brain homogenate, neuronal cell lysate, 40 ug
    Results: Very weak band at 25 kDa

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry that ab109236 is not producing a strong 25 kDa band in Western blot for your mouse brain homogenate. As we discussed on the phone, I am sending you a positive control free of charge. I also recommend attempting a primary dilution of 1/500 in case your samples simply contain a low amount of Neurogenin 2. Protease and phosphatase inhibitors should also be added if you have not already done so. Additionally, since the Neurogenin 2 protein is localized to the nucleus we recommend using RIPA buffer.

    Our western blot protocol along with troubleshooting tips is given through the link below:
    https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    We initially discussed sending a HepG2 (liver carcinoma) cell lysate as the positive control, but after doing further searching I found a U-87 MG Nuclear Lysate (ab14903: human glioblastoma-astrocytoma) that has been used as a positive control for Neurogenin 2 and is more relavant to your research being a brain cell line as you desired.

    Your order # is **** for ab14903, and will ship Monday for Tuesday delivery.

    Please do not hesitate to contact us if you need any more advice or information, the positive control will tell us if the antibody is properly detected its antigen or not.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com