RecombinantRabMAb

Recombinant Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

Reviews (1)Q&A (1)
Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
  • Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neurogenin 2/NGN2 antibody [EPR4177]
    See all Neurogenin 2/NGN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4177] to Neurogenin 2/NGN2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Neurogenin 2/NGN2 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H2A3
    (Peptide available as ab187850)

  • Positive control

    • WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.

  • General notes

     

     

     This product was previously labelled as Neurogenin 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    Images

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)

      Lane 1 : PC-12 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 29 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 29 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Western blot - Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      All lanes : Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)
      Anti-Neurogenin 2/NGN2 antibody [EPR4177] (ab109236)

    References (0)

    Publishing research using ab109236? Please let us know so that we can cite the reference in this datasheet.

    ab109236 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Neurogenin 2/NGN2 antibody [EPR4177]

     Poor
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Retina)
    Permeabilization
    Yes - 0.3% triton
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 21 2014

    Question

    Customer kindly contacted us that ab109236 has not been giving a strong band in western blot.
    Application: Western blot
    Primary: 1/1000
    Block: milk
    Sample: mouse brain homogenate, neuronal cell lysate, 40 ug
    Results: Very weak band at 25 kDa

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry that ab109236 is not producing a strong 25 kDa band in Western blot for your mouse brain homogenate. As we discussed on the phone, I am sending you a positive control free of charge. I also recommend attempting a primary dilution of 1/500 in case your samples simply contain a low amount of Neurogenin 2. Protease and phosphatase inhibitors should also be added if you have not already done so. Additionally, since the Neurogenin 2 protein is localized to the nucleus we recommend using RIPA buffer.

    Our western blot protocol along with troubleshooting tips is given through the link below:
    https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    We initially discussed sending a HepG2 (liver carcinoma) cell lysate as the positive control, but after doing further searching I found a U-87 MG Nuclear Lysate (ab14903: human glioblastoma-astrocytoma) that has been used as a positive control for Neurogenin 2 and is more relavant to your research being a brain cell line as you desired.

    Your order # is **** for ab14903, and will ship Monday for Tuesday delivery.

    Please do not hesitate to contact us if you need any more advice or information, the positive control will tell us if the antibody is properly detected its antigen or not.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com