Overview

  • Product name

    Anti-Neuroglycan C antibody
    See all Neuroglycan C primary antibodies
  • Description

    Rabbit polyclonal to Neuroglycan C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse Neuroglycan C.

    Read Abcam's proprietary immunogen policy (Peptide available as ab32755.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue Lysate Spinal Cord (Rat) Lysate. Brain (Mouse) Tissue Lysate Brain (Rat) Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab31946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-FoFr
WB
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-FoFr: 1/300 (See abreview for more details)
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60,45 kDa (predicted molecular weight: 60,58,48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
    • Tissue specificity

      Restricted to brain (at protein level).
    • Sequence similarities

      Contains 1 EGF-like domain.
    • Developmental stage

      Expressed in brain of 3 months, 5 and 10-year-old individuals.
    • Post-translational
      modifications

      N-glycosylated.
      O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation.
      Phosphorylated; in intracellular and extracellular parts.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • Acidic leucine rich EGF like domain containing brain protein antibody
      • Acidic leucine-rich EGF-like domain-containing brain protein antibody
      • Caleb antibody
      • Chondroitin sulfate proteoglycan 5 (neuroglycan C) antibody
      • Chondroitin sulfate proteoglycan 5 antibody
      • CSPG5 antibody
      • CSPG5_HUMAN antibody
      • MGC44034 antibody
      • Neuroglycan C antibody
      • Ngc antibody
      • OTTHUMP00000164796 antibody
      • OTTHUMP00000164797 antibody
      • OTTHUMP00000210429 antibody
      see all

    Images

    • ab31946 staining Neuroglycan C in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

      This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

    • All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml

      Lane 1 : Mouse Spinal Cord Lysate
      Lane 2 : Rat Spinal Cord Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 60,58,48 kDa
      Observed band size: 45,60 kDa
      why is the actual band size different from the predicted?



      It is impossible to conclude from the data whether the higher observed band is Neuroglycan C isoform 3 (60 kDa) or Neuroglycan C isoform 1 (57 kDa).  The epitope for this antibody is present in all 3 isoforms of the Neuroglycan C protein. We suspect the lower band is Isoform 2.
    • All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 60,58,48 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • ICC/IF image of ab31946 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31946, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    References

    This product has been referenced in:

    • Finelli MJ  et al. The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons. Hum Mol Genet 28:584-597 (2019). Read more (PubMed: 30335140) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No

    Dr. Sophie Pezet

    Verified customer

    Submitted Jun 05 2009

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