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RecombinantRabMAb

Recombinant Anti-Neurogranin antibody [EPR21152] (ab217672)

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  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Western blot - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] (ab217672)
  • Anti-Neurogranin antibody [EPR21152] (ab217672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21152] to Neurogranin
  • Suitable for: IHC-Fr, WB, IHC-P, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Primary
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Anti-SNAP25 antibody [EPR3275] (ab109105)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human Neurogranin protein (ab181939)

View more associated products

Overview

  • Product name

    Anti-Neurogranin antibody [EPR21152]
    See all Neurogranin primary antibodies
  • Description

    Rabbit monoclonal [EPR21152] to Neurogranin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain lysate and brain cortex lysate; mouse cerebral cortex lysate. Rat brain lysate. IHC-P: Human cerebellum tissue; mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat hippocampus CA1 tissue. IP: Mouse cerebral cortex lysate. ICC/IF: Primary rat cortical neurons
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21152
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Neuroscience
    • Neurology process
    • Neurogenesis

Associated products

  • Alternative Versions

    • Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Neurogranin protein (ab181939)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
IHC-Fr
Mouse
Rat
IHC-P
Mouse
Rat
Human
IP
Mouse
WB
Mouse
Rat
Human
Application Abreviews Notes
IHC-Fr
1/3000.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
ICC/IF
1/1500.
Notes
IHC-Fr
1/3000.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
ICC/IF
1/1500.

Target

  • Function

    Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • Tissue specificity

    In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • Sequence similarities

    Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Target information above from: UniProt accession Q92686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4900 Human
    • Entrez Gene: 64011 Mouse
    • Entrez Gene: 64356 Rat
    • Omim: 602350 Human
    • SwissProt: Q92686 Human
    • SwissProt: P60761 Mouse
    • SwissProt: Q04940 Rat
    • Unigene: 524116 Human
    • Unigene: 335065 Mouse
    • Unigene: 11236 Rat
    see all
  • Alternative names

    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on rat cerebrum (PMID: 26076492) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Western blot - Anti-Neurogranin antibody [EPR21152] (ab217672)
    All lanes : Anti-Neurogranin antibody [EPR21152] (ab217672) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate at 10 µg
    Lane 2 : Human brain cortex lysate at 10 µg
    Lane 3 : Mouse cerebral cortex lysate at 20 µg
    Lane 4 : Rat brain lysate at 20 µg

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 8 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 32 seconds

    Lane 2: 101 seconds

    Lane 3: 85 seconds

    Lane 4: 3 minutes 

    The observed molecular mass is consistent with the literature (PMID 17505539).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus CA1 tissue labeling Neurogranin with ab217672 at 1/3000 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on mouse brain (PMID: 15389631; 21516261) is observed. Counter stained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunocytochemistry/ Immunofluorescence - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunocytochemistry/ Immunofluorescence - Anti-Neurogranin antibody [EPR21152] (ab217672)Image courtesy of Ms. Babben Tinner, QBM Cell Science.

    Immunocytochemistry/ Immunofluorescence analysis of primary rat cortical neurons labeling Neurogranin with ab217672 at 1/1500. The cells were fixed with 4% paraformaldehyde containing 0.2% picric acid in 0.1M phosphate buffer, pH 6.9 for 20 minutes. Permeabilization was with 0.3% Triton-X 100 in PBS (PBSTx). ab150068 at 1/200 was used as the secondary antibody

    The rat cortical neurons were cultured for 29 days in vitro. They were either left untreated (Control) or treated beginning on the 10th day with 60ng/mL triiodothyronine (T3), to enhance neurogranin expression.

    The cells were visualized with an inverted microscope at 10X magnification (upper panels) or 20X magnification (lower panels).

  • Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized  frozen rat hippocampus CA1 tissue labeling Neurogranin with ab217672 at 1/3000 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on rat brain (PMID: 15389631; 21516261) is observed. Counter stained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on mouse cerebrum (PMID: 26076492) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic but also weak nuclear staining on human cerebrum (PMID: 26076492; PMID: 21516261) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] (ab217672)
    Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] (ab217672)

    Neurogranin was immunoprecipitated from 0.35 mg mouse cerebral cortex lysate with ab217672 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217672 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

    Lane 1: Mouse cerebral cortex lysate 10 µg (Input).
    Lane 2: ab217672 IP in mouse cerebral cortex lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217672 in mouse cerebral cortex lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

  • Anti-Neurogranin antibody [EPR21152] (ab217672)
    Anti-Neurogranin antibody [EPR21152] (ab217672)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab217672? Please let us know so that we can cite the reference in this datasheet.

ab217672 has not yet been referenced specifically in any publications.

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