Recombinant Anti-Neurogranin antibody [Ng2] (ab273443)
![Western blot - Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-409543-AntiNeurogranin-antibody-Ng2-western-blot-brain-hippocampus-human-mouse-rat.jpg "Western blot - Anti-Neurogranin antibody [Ng2] (ab273443)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Ng2] to Neurogranin
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Neurogranin antibody [Ng2]
See all Neurogranin primary antibodies -
Description
Mouse monoclonal [Ng2] to Neurogranin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: Human brain tissue lysate; Mouse hippicampus tissue lysate; Rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IP: Mouse hippocampus tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Ng2 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
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| IP |
1/30.
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| Notes |
|---|
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IHC-P
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). |
|
IP
1/30. |
Target
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Function
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. -
Tissue specificity
In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease. -
Sequence similarities
Belongs to the neurogranin family.
Contains 1 collagen-like domain.
Contains 1 IQ domain. - Information by UniProt
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Database links
- Entrez Gene: 4900 Human
- Entrez Gene: 64011 Mouse
- Entrez Gene: 64356 Rat
- Omim: 602350 Human
- SwissProt: Q92686 Human
- SwissProt: P60761 Mouse
- SwissProt: Q04940 Rat
- Unigene: 524116 Human
see all -
Alternative names
- Protein kinase C substrate RC3 antibody
- Calmodulin binding protein antibody
- Hng antibody
see all
Images
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Western blot - Anti-Neurogranin antibody [Ng2] (ab273443)All lanes : Anti-Neurogranin antibody [Ng2] (ab273443) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse hippocampus tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat hippocampus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 8 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Negative control: Mouse spleen (PMID: 20665622).
The molecular weight observed is consistent with what has been described in the literature ( PMID: 20875798).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 secs; Lanes 2-4: 3 secs.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-409544-AntiNeurogranin-antibody-Ng2-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
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![Immunoprecipitation - Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-409549-AntiNeurogranin-antibody-Ng2-immunoprecipitation-hippocampus-mouse.jpg)
Immunoprecipitation - Anti-Neurogranin antibody [Ng2] (ab273443)Neurogranin was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with ab273443 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273443 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.
Lane 1: Mouse hippocampus tissue lysate 10µg.
Lane 2: ab273443 IP in mouse hippocampus tissue lysate.
Lane 3: Mouse monoclonal IgG2b instead of ab273443 in Mouse hippocampus tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 58 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-409545-AntiNeurogranin-antibody-Ng2-immunohistochemistry-cerebrum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-409547-AntiNeurogranin-antibody-Ng2-immunohistochemistry-cerebrum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
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![Anti-Neurogranin antibody [Ng2] (ab273443)](https://www.abcam.com/ps/products/273/ab273443/Images/ab273443-6-benefits-of-recombinant-antibodies.png)
Anti-Neurogranin antibody [Ng2] (ab273443)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab273443 has not yet been referenced specifically in any publications.
