Product nameNeuroinflammation Marker (BDNF, ICAM1, TREM2, GFAP, TNF alpha, Iba1) Antibody Panel - Human
Species reactivityReacts with: Human
Neuroinflammation Marker (BDNF, ICAM1, TREM2, GFAP, TNF alpha, Iba1) Antibody Panel - Human ab263462 contains multiple trial-sized versions of anti-human antibody clones against BDNF, ICAM1, TREM2, GFAP, TNF alpha, Iba1, specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [EPR1292] to BDNF (20 µL) ab108319
- Rabbit monoclonal [EP1442Y] to ICAM1 (20 µL) ab53013
- Rabbit monoclonal [EPR20243] to TREM2 (20 µL) ab209814
- Rabbit monoclonal [EP672Y] to GFAP (20 µL) ab33922
- Rabbit monoclonal [EPR20972] to TNF alpha (20 µL) ab215188
- Rabbit monoclonal [EPR16589] to Iba1 (20 µL) ab178847
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab108319 - Anti-BDNF antibody [EPR1292] 2 x 10µl ab33922 - Anti-GFAP antibody [EP672Y] 2 x 10µl ab178847 - Anti-Iba1 antibody [EPR16589] 2 x 10µl ab53013 - Anti-ICAM1 antibody [EP1442Y] 2 x 10µl ab215188 - Anti-TNF alpha antibody [EPR20972] 2 x 10µl ab209814 - Anti-TREM2 antibody [EPR20243] 2 x 10µl
Cellular localizationICAM1: Membrane. TNF alpha: Secreted and Cell membrane. GFAP: Cytoplasm. Associated with intermediate filaments. BDNF: Secreted. Iba1: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. TREM2: Secreted and Cell membrane.
- Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
- Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
- Anti-ICAM1 antibody [EP1442Y] - Low endotoxin, Azide free (ab221777)
- Anti-Iba1 antibody [EPR16589] - BSA and Azide free (ab221790)
- Alexa Fluor® 488 Anti-BDNF antibody [EPR1292] (ab222113)
- Alexa Fluor® 647 Anti-BDNF antibody [EPR1292] (ab222321)
- Anti-TNF alpha antibody [EPR20972] - BSA and Azide free (ab225576)
- Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)
- Alexa Fluor® 488 Anti-TNF alpha antibody [EPR20972] (ab237353)
- Alexa Fluor® 647 Anti-TNF alpha antibody [EPR20972] (ab237354)
- PE Anti-TNF alpha antibody [EPR20972] (ab237355)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized U937 (Human histiocytic lymphoma cell line) cells labeling Iba1 with ab178847 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on U937 cell line.
The nuclear counterstain is DAPI (blue).
The negative controls are as follows:-
Cytoplasm staining on microglia of the normal Human cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
ab33922 showing positive staining in Normal brain tissue.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-N-F1 cells labeling TREM2 with ab209814 at 1/500 dilution, followed by Donkey anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (green).
The nuclear counter stain is DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ICAM1 with ab53013 at 1/1000. Heat mediated antigen retrieval was performed using ab93684, Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
All lanes : Anti-TNF alpha antibody [EPR20972] (ab215188) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) differentiated with 100 nM TPA overnight, whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) differentiated with 100 nM TPA overnight, then treated with 100 ng/ml lipopolysaccharides (LPS) for 7 hours with addition of 1 µg/ml brefeldin A (BFA) for the last 3 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263462 has not yet been referenced specifically in any publications.