Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neurokinin A antibody

Immunohistochemistry (PFA perfusion fixed frozen sections)

Mouse Tissue sections (Brain)

No

Brain

Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

Zamboni

12 hour(s) and 0 minute(s) · Temperature: 4°C

1/50

1/400

Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L)Cyanine Cy3  
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Primary Antibody Dilution: 1:50, 1:100 in 5% normal donkey serum Incubation time & temperature: 1h at RT and then 12h at 4oC Secondary Antibody Dilution: 1:400 in 5% normal donkey serum Incubation Time & Temperature: 2h, RT

We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.