Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Zamboni
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L)Cyanine Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Primary Antibody
Dilution: 1:50, 1:100 in 5% normal donkey serum
Incubation time & temperature: 1h at RT and then 12h at 4oC
Secondary Antibody
Dilution: 1:400 in 5% normal donkey serum
Incubation Time & Temperature: 2h, RT
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
Mr. Duc Dung Le
Verified customer
Submitted Apr 29 2015