Neurokinin A, Tachykinin peptide (ab120185)
Key features and details
- Tachykinin peptide
- CAS Number: 86933-74-6
- Soluble in water
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Neurokinin A, Tachykinin peptide -
Description
Tachykinin peptide -
Alternative names
- NKA
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Biological description
Endogenous tachykinin peptide
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CAS Number
86933-74-6 -
Chemical structure
Properties
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Molecular weight
1133.32 -
Molecular formula
C50H80N14O14S -
Sequence
HKTDSFVGLM (Modifications: C-terminal amide) -
PubChem identifier
55582 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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2D chemical structure image of ab120185, Neurokinin A, Tachykinin peptide
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K562 cells were incubated at 37°C for 4h with vehicle control (0 μM) and 15 nM of neurokinin A (ab120185). Increased expression of p53 (ab26) correlates with an increase in neurokinin A concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab26 at 5 μg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120185 has been referenced in 1 publication.
- Drimousis S et al. Gender-Related Differences of Tachykinin NK2 Receptor Expression and Activity in Human Colonic Smooth Muscle. J Pharmacol Exp Ther 375:28-39 (2020). PubMed: 32764152