Key features and details
- Mouse monoclonal [N97A/31] to Neuroligin 1
- Suitable for: IHC, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Neuroligin 1 antibody [N97A/31]
See all Neuroligin 1 primary antibodies
DescriptionMouse monoclonal [N97A/31] to Neuroligin 1
Specificityab186279 does not cross-react with other Neuroligins.
Tested applicationsSuitable for: IHC, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein (His-tag) corresponding to Rat Neuroligin 1 aa 718-843. Cytoplasmic domain.
Database link: Q62765
- WB: Mouse brain membrane lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S97A-31 to N97A/31, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration.|
|WB||1/500. Predicted molecular weight: 94 kDa.|
FunctionNeuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses.
Tissue specificityExpressed in the blood vessel walls (at protein level).
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Cellular localizationCell membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities.
- Information by UniProt
- NLG 1 antibody
- KIAA1070 antibody
- MGC45115 antibody
SK-N-BE (human neuroblastoma cell line) cells stained for Neuroligin 1 (green) using ab186279 at 1/100 dilution in ICC/IF. The secondary antibody is Goat Anti-Mouse ATTO 488. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red (red).
Anti-Neuroligin 1 antibody [N97A/31] (ab186279) at 1/200 dilution + Mouse brain membrane lysate
Goat Anti-Mouse IgG-HRP at 1/1000 dilution
Predicted band size: 94 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186279 has been referenced in 2 publications.
- Tian M et al. NL1 expression level in Nrx1ß and the excitability of PV interneurons in mice with POCD. Exp Ther Med 17:3117-3123 (2019). PubMed: 30936983
- Zhao ZH et al. Low-level Gestational Lead Exposure Alters Dendritic Spine Plasticity in the Hippocampus and Reduces Learning and Memory in Rats. Sci Rep 8:3533 (2018). PubMed: 29476096