Overview

  • Product name

    Anti-Neuroligin 1 antibody [N97A/31]
    See all Neuroligin 1 primary antibodies
  • Description

    Mouse monoclonal [N97A/31] to Neuroligin 1
  • Host species

    Mouse
  • Specificity

    ab186279 does not cross-react with other Neuroligins.
  • Tested applications

    Suitable for: IHC, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Rat Neuroligin 1 aa 718-843. Cytoplasmic domain.
    Database link: Q62765

  • Positive control

    • WB: Mouse brain membrane lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S97A-31 to N97A/31, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab186279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 94 kDa.
ICC/IF 1/100.

Target

  • Function

    Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses.
  • Tissue specificity

    Expressed in the blood vessel walls (at protein level).
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization

    Cell membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities.
  • Information by UniProt
  • Database links

  • Alternative names

    • NLG 1 antibody
    • KIAA1070 antibody
    • MGC45115 antibody
    • Neuroligin-1 antibody
    • NL1 antibody
    • NLG1 antibody
    • Nlgn1 antibody
    • NLGN1_HUMAN antibody
    see all

Images

  • SK-N-BE (human neuroblastoma cell line) cells stained for Neuroligin 1 (green) using ab186279 at 1/100 dilution in ICC/IF. The secondary antibody is Goat Anti-Mouse ATTO 488. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red (red).

  • Anti-Neuroligin 1 antibody [N97A/31] (ab186279) at 1/200 dilution + Mouse brain membrane lysate

    Secondary
    Goat Anti-Mouse IgG-HRP at 1/1000 dilution

    Predicted band size: 94 kDa

References

This product has been referenced in:

  • Tian M  et al. NL1 expression level in Nrx1ß and the excitability of PV interneurons in mice with POCD. Exp Ther Med 17:3117-3123 (2019). Read more (PubMed: 30936983) »
  • Zhao ZH  et al. Low-level Gestational Lead Exposure Alters Dendritic Spine Plasticity in the Hippocampus and Reduces Learning and Memory in Rats. Sci Rep 8:3533 (2018). Read more (PubMed: 29476096) »
See all 2 Publications for this product

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