RecombinantRabMAb

Recombinant Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36] (ab259936)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
  • Western blot - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit Monoclonal [EPR24149-36] to Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36]

  • Description

    Rabbit Monoclonal [EPR24149-36] to Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Neuroligin 1 aa 1-650. The exact sequence is proprietary.
    Database link: Q99K10

  • Positive control

    • IHC-P: Human cerebrum and astrocytoma tissue; Mouse cerebrum tissue; Rat cerebrum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab259936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC,IP or WB.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is  ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human astrocytoma. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID: 7736595). The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 1 + Neuroligin 2 + Neuroligin 3 + Neuroligin 4 with ab259936 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on rat liver (PMID: 7736595). The section was incubated with ab259936 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      Western blot - Anti-Neuroligin 1 + Neuroligin 3 + Neuroligin 2 + Neuroligin 4 antibody [EPR24149-36] (ab259936)
      All lanes : Anti-Neuroligin 1+Neuroligin 2+Neuroligin 3+Neuroligin 4 antibody [EPR24149-36] (ab259936) at 1/1000 dilution

      Lane 1 : His-tagged mouse neuroligin 1 recombinant protein, 10 ng
      Lane 2 : LIF/His-tagged mouse neuroligin 2 recombinant protein, 10 ng
      Lane 3 : His-tagged mouse neuroligin 3 recombinant protein, 10 ng
      Lane 4 : His-tagged human neuroligin 4 recombinant protein, 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The rec proteins of neuroligin 1, neuroligin 2 and neuroligin 3 were made in house.

      The recombinant proteins were expressed from a mammalian - HEK-293 expression system.

      Exposure time: 3 minutes.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab259936? Please let us know so that we can cite the reference in this datasheet.

    ab259936 has not yet been referenced specifically in any publications.

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