Overview

  • Product name

    Anti-Neuron specific beta III Tubulin antibody
  • Description

    Rabbit polyclonal to Neuron specific beta III Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Neuron specific beta III Tubulin (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q13509

  • Positive control

    • WB: HeLa, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; Mouse and rat brain tissue extracts. IP: SK-N-SH whole cell extract. IHC-P: Rat brain tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab229590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/50000.
IHC-Fr 1/100 - 1/1000.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • Tissue specificity

    Expression is primarily restricted to central and peripheral nervous system.
  • Involvement in disease

    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • beta 4 antibody
    • MC1R antibody
    • TBB3_HUMAN antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    see all

Images

  • All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Neuron specific beta III Tubulin Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG
  • All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Neuron specific beta III Tubulin knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.


    10% SDS-PAGE gel.

  • Frozen E13.5 rat brain tissue stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/250 dilution in immunohistochemical analysis.

    Nuclear counterstain: Fluoroshield with DAPI (blue).

  • 4 % paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Fluoroshield with DAPI (blue).

  • Paraffin-embedded rat brain tissue stained for Neuron specific beta III Tubulin using ab229590 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.


    10% SDS-PAGE gel.

  • All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/50000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.


    10% SDS-PAGE gel.

  • Neuron specific beta III Tubulin was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229590. Western blot was performed from the immunoprecipitate using ab229590. Anti-rabbit IgG was used as a secondary antibody.

    Lane 1: SK-N-SH whole cell extract (Input).
    Lane 2: Control IgG instead of ab229590 in SK-N-SH whole cell extract.
    Lane 3: ab229590 IP in SK-N-SH whole cell extract.

References

This product has been referenced in:

  • Stewart AN  et al. Transplantation of Mesenchymal Stem Cells that Overexpress NT-3 Produce Motor Improvements without Axonal Regeneration following Complete Spinal Cord Transections in Rats. Brain Res N/A:N/A (2018). IHC-P . Read more (PubMed: 29883625) »
  • Bao N  et al. Upregulation of miR-199a-5p Protects Spinal Cord Against Ischemia/Reperfusion-Induced Injury via Downregulation of ECE1 in Rat. Cell Mol Neurobiol 38:1293-1303 (2018). Read more (PubMed: 29948551) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229590.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up