Neuron specific Enolase Antibody Pair - BSA and Azide free (ab244189)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Human
Overview
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Product name
Neuron specific Enolase Antibody Pair - BSA and Azide free
See all NSE kits -
Assay type
ELISA set -
Range
312.5 pg/ml - 20000 pg/ml -
Species reactivity
Reacts with: Mouse, Human -
Product overview
The Antibody Pair can be used to quantify Human Neuron specific Enolase. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab217778), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Neuron specific Enolase Capture Antibody (unconjugated) 1 x 100µg Neuron specific Enolase Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Alternative names
- 2 phospho D glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- Eno 2
see all -
Database links
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- Unigene: 511915 Human
- Unigene: 3913 Mouse
Associated products
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Affibody® Molecule
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Neuron specific Enolase Antibody Pair - BSA and Azide free (ab244189)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217778), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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Sandwich ELISA - Neuron specific Enolase Antibody Pair - BSA and Azide free (ab244189)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217778), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits. -
Sandwich ELISA - Neuron specific Enolase Antibody Pair - BSA and Azide free (ab244189)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217778), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits. -
Sandwich ELISA - Neuron specific Enolase Antibody Pair - BSA and Azide free (ab244189)To learn more about the advantages of recombinant antibodies see here.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab244189 has not yet been referenced specifically in any publications.
