Anti-Neuronatin antibody (ab27266)
- Datasheet
- References (14)
- Protocols
Overview
-
Product name
Anti-Neuronatin antibody
See all Neuronatin primary antibodies -
Description
Rabbit polyclonal to Neuronatin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Neuronatin.
Read Abcam's proprietary immunogen policy (Peptide available as ab30332.) -
Positive control
- Mouse Brain Whole Tissue Lysate (0-days old). SKNSH cell line (ICC/IF).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab27266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22624064 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20872847 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). Abcam recommends using 1-3% Milk as the blocking agent. |
|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. PubMed: 21742036 |
Target
-
Function
May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels. -
Sequence similarities
Belongs to the neuronatin family. -
Developmental stage
Abundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood. - Information by UniProt
-
Database links
- Entrez Gene: 4826 Human
- Entrez Gene: 18111 Mouse
- Entrez Gene: 94270 Rat
- Omim: 603106 Human
- SwissProt: Q16517 Human
- SwissProt: Q61979 Mouse
- SwissProt: Q62649 Rat
- Unigene: 504703 Human
see all -
Alternative names
- Neuronatin antibody
- NNAT antibody
- NNAT_HUMAN antibody
see all
Images
-
Western blot - Anti-Neuronatin antibody (ab27266)Anti-Neuronatin antibody (ab27266) at 1 µg/ml +Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa -
Western blot - Anti-Neuronatin antibody (ab27266)Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (blocked with 3% Milk) at 50 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 12 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuronatin antibody (ab27266)This image is courtesy of an anonymous Abreviewab27266 at 1/1000 staining human pituitary cells by IHC-P The antibody was tested on normal human pituitary slide sections (formalin fixed/paraffin embedded). This gave good staining over a range of concentrations with a microwave/pressure antigen retrieval step. Staining was strongly associated with the cell membrane and blocked by incubating with the neuronatin antibody with the peptide which the antibody was raised against. -
Western blot - Anti-Neuronatin antibody (ab27266)Anti-Neuronatin antibody (ab27266) at 1 µg/ml +Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 70-75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Neuronatin antibody (ab27266)ICC/IF image of ab27266 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuronatin antibody (ab27266)Image from Wagnon JL et al., PLoS Genet 8(11): e1003067.. Fig 6.; doi: 10.1371/journal.pgen.1003067. Epub 2012 Nov 29.Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Neuronatin with ab27266. Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Vatsa N et al. Down-Regulation of miRNA-708 Promotes Aberrant Calcium Signaling by Targeting Neuronatin in a Mouse Model of Angelman Syndrome. Front Mol Neurosci 12:35 (2019). Read more (PubMed: 30814928) »
- Millership SJ et al. Neuronatin regulates pancreatic ß cell insulin content and secretion. J Clin Invest 128:3369-3381 (2018). Read more (PubMed: 29864031) »
