For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    neuronatin-antibody-ab27266.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Metabolism
Share by email

Anti-Neuronatin antibody (ab27266)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (18)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Neuronatin antibody (ab27266)
  • Western blot - Anti-Neuronatin antibody (ab27266)
  • Western blot - Anti-Neuronatin antibody (ab27266)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuronatin antibody (ab27266)

Key features and details

  • Rabbit polyclonal to Neuronatin
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Neuronatin antibody [EPR13554(B)] (ab181353)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Neuronatin antibody
    See all Neuronatin primary antibodies
  • Description

    Rabbit polyclonal to Neuronatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Metabolism
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).

Abcam recommends using 1-3% Milk as the blocking agent.

Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).

Abcam recommends using 1-3% Milk as the blocking agent.

Target

  • Function

    May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels.
  • Sequence similarities

    Belongs to the neuronatin family.
  • Developmental stage

    Abundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood.
  • Target information above from: UniProt accession Q16517 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4826 Human
    • Entrez Gene: 18111 Mouse
    • Entrez Gene: 94270 Rat
    • Omim: 603106 Human
    • SwissProt: Q16517 Human
    • SwissProt: Q61979 Mouse
    • SwissProt: Q62649 Rat
    • Unigene: 504703 Human
    • Unigene: 34330 Mouse
    • Unigene: 5785 Rat
    see all
  • Alternative names

    • Neuronatin antibody
    • NNAT antibody
    • NNAT_HUMAN antibody
    • Peg 5 antibody
    • Peg5 antibody
    see all

Images

  • Western blot - Anti-Neuronatin antibody (ab27266)
    Western blot - Anti-Neuronatin antibody (ab27266)
    Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa

  • Western blot - Anti-Neuronatin antibody (ab27266)
    Western blot - Anti-Neuronatin antibody (ab27266)
    Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (blocked with 3% Milk) at 50 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 9 kDa
    Observed band size: 9 kDa


    Exposure time: 12 minutes
  • Western blot - Anti-Neuronatin antibody (ab27266)
    Western blot - Anti-Neuronatin antibody (ab27266)
    Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa
    Observed band size: 10 kDa why is the actual band size different from the predicted?
    Additional bands at: 70-75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuronatin antibody (ab27266)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuronatin antibody (ab27266)Image from Wagnon JL et al., PLoS Genet 8(11): e1003067.. Fig 6.; doi: 10.1371/journal.pgen.1003067. Epub 2012 Nov 29.
    Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Neuronatin with ab27266. Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab27266? Please let us know so that we can cite the reference in this datasheet.

ab27266 has been referenced in 18 publications.

  • Sinha P  et al. Trehalose Ameliorates Seizure Susceptibility in Lafora Disease Mouse Models by Suppressing Neuroinflammation and Endoplasmic Reticulum Stress. Mol Neurobiol 58:1088-1101 (2021). PubMed: 33094475
  • Saeed H  et al. Aberrant epigenetic silencing of neuronatin is a frequent event in human osteosarcoma. Oncotarget 11:1876-1893 (2020). PubMed: 32499872
  • Kanno N  et al. Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas. J Histochem Cytochem 67:495-509 (2019). PubMed: 30869556
  • Vatsa N  et al. Down-Regulation of miRNA-708 Promotes Aberrant Calcium Signaling by Targeting Neuronatin in a Mouse Model of Angelman Syndrome. Front Mol Neurosci 12:35 (2019). PubMed: 30814928
  • Plasterer C  et al. Neuronatin is a modifier of estrogen receptor-positive breast cancer incidence and outcome. Breast Cancer Res Treat 177:77-91 (2019). PubMed: 31165373
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Neuronatin antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Geniculate ganglion and hippocampus)
Permeabilization
Yes - 1% triton for 20 minutes
Specification
Geniculate ganglion and hippocampus
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuronatin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (white adipose)
Specification
white adipose
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, 10mM pH6
Permeabilization
Yes - 0.4% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 25 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuronatin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Cell (pituitary)
Specification
pituitary
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 22 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.