Key features and details
- Rabbit polyclonal to Neuropeptide S
- Suitable for: IHC (PFA fixed), RIA
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Neuropeptide S antibody
See all Neuropeptide S primary antibodies
DescriptionRabbit polyclonal to Neuropeptide S
SpecificityThis antibody is generated against the complete 20aa sequence of the fully processed rat NPS peptide, it should run at about 2kDa on WB. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue.
Tested applicationsSuitable for: IHC (PFA fixed), RIAmore details
Species reactivityReacts with: Mouse, Rat
- Rat and Mouse brain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab18252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)||Use a concentration of 0.3 µg/ml.|
|RIA||Use a concentration of 0.1 - 0.3 µg/ml.|
FunctionModulates arousal and anxiety. May play an important anorexigenic role (By similarity). Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations (PubMed:15312648, PubMed:16790440).
- Information by UniProt
- Neuropeptide S antibody
- Nps antibody
- NPS_HUMAN antibody
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).
Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
ab18252 has been referenced in 6 publications.
- Tillmann S et al. Sustained overexpression of neuropeptide S in the amygdala reduces anxiety-like behavior in rats. Behav Brain Res 367:28-34 (2019). PubMed: 30914309
- Breton-Provencher V & Sur M Active control of arousal by a locus coeruleus GABAergic circuit. Nat Neurosci 22:218-228 (2019). IHC-FoFr ; Mouse . PubMed: 30643295
- Grund T et al. Neuropeptide S Activates Paraventricular Oxytocin Neurons to Induce Anxiolysis. J Neurosci 37:12214-12225 (2017). Rat . PubMed: 29118105
- Adori C et al. Neuropeptide S- and Neuropeptide S receptor-expressing neuron populations in the human pons. Front Neuroanat 9:126 (2015). IHC ; Rat . PubMed: 26441556
- Liu X et al. Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain. J Comp Neurol 519:1847-66 (2011). Mouse . PubMed: 21452230
- Clark SD et al. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol 519:1867-93 (2011). Mouse . PubMed: 21452235