Overview

  • Product name

    Anti-Neuropeptide Y antibody [8]
    See all Neuropeptide Y primary antibodies
  • Description

    Mouse monoclonal [8] to Neuropeptide Y
  • Host species

    Mouse
  • Specificity

    Cross-reactivity with Neuropeptide Y(1-24), Neuropeptide Y (18-36), Peptide YY or Pancreatic Polypeptide was <0.2%.
  • Tested applications

    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human/Rat Neuropeptide Y adsorbed onto aluminum hydroxide gel.

  • Epitope

    The epitope of ab112473 is probably around the mid-molecular region of Neuropeptide Y.
  • Positive control

    • Mouse brain tissue (fibres in the hilus of the hippocampus, following epiletic attack) Mouse brain tissue (interneurons in the cortex)

Properties

Applications

Our Abpromise guarantee covers the use of ab112473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/1000.

Target

Images

  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion by Immunohistochemistry.
  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion by Immunohistochemistry.
  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (interneurons in the cortex) by Immunohistochemistry.
  • Immunofluorescence images of  tissue from human cerebral cortex, applying ab112473 to ultra thin sections (antibody: green,

    DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil.

  • Immunofluorescence images of  tissue from human cerebral cortex, applying ab112473 to ultra thin sections (antibody: green,

    DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil.

References

This product has been referenced in:

  • Abbineni PS  et al. Chromogranin A, the major lumenal protein in chromaffin granules, controls fusion pore expansion. J Gen Physiol 151:118-130 (2019). Read more (PubMed: 30504267) »
  • Harris KD  et al. Classes and continua of hippocampal CA1 inhibitory neurons revealed by single-cell transcriptomics. PLoS Biol 16:e2006387 (2018). Read more (PubMed: 29912866) »
See all 5 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde

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Verified customer

Submitted Sep 02 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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