Overview

  • Product name
    Anti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description
    Rabbit polyclonal to Neuropeptide Y
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, IHC-P, Dot blot, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Monkey
  • Immunogen

    Full length Neuropeptide Y (porcine) conjugated to KLH.

  • Positive control
    • NPY-containing neurons (cell bodies, axons, and dendrites) in frozen sections of rat brain obtained following perfusion fixation with 4% paraformaldehyde. Formalin-fixed, frozen, or Vibratome sections of rat, cat, porcine, bovine, monkey, or human brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab10980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17455332Use at an assay dependent dilution. PMID 17455332.
ICC/IF Use at an assay dependent concentration. PubMed: 19609763
IHC-P 1/800.
Dot blot 1/10000. 1/10000. This concentration was determined using Neuropeptide Y-BSA (0.03-0.25 ng/dot).
IHC-Fr 1/8000. 1/8000. This concentration was determined using frozen sections of rat brain perfusion-fixed with 4% paraformaldehyde. Absorption studies show that specific staining of tissue may be inhibited following preincubation of the antiserum with 10 µM neuropeptide Y (porcine).
RIA 1/10000. 1/10000. This concentration was determined using 20 pg/tube of 125I-labeled neuropeptide Y in a second antibody and polyethylene glycol RIA. The sensitivity of the RIA assay is defined as the 90% intercept of a B/B0 standard curve. In this system the sensitivity has been found to be 20 pg/tube. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = Minimum 5.0 x 1010 L/mole

Target

Images

  • ab10980 staining rat brain, striatum tissue sections by IHC-P.  Sections were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% serum for 2 hours at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 14 hours at 20°C.  Arrows point to Neuropeptide Y positive interneurons. Magnification 20X.

    See Abreview

  • ab10980 staining Neuropeptide Y in chicken brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed with a Tris-EDTA buffer, pH 9. Samples were then permeabilized using 0.1% Triton X-100/PBS, blocked with 4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS for 1 hour at 4°C and then incubated with ab10980 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:
  • Niquille M  et al. Neurogliaform cortical interneurons derive from cells in the preoptic area. Elife 7:N/A (2018). Read more (PubMed: 29557780) »
  • Puighermanal E  et al. Anatomical and molecular characterization of dopamine D1 receptor-expressing neurons of the mouse CA1 dorsal hippocampus. Brain Struct Funct 222:1897-1911 (2017). Read more (PubMed: 27678395) »
See all 15 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

I am sorry for the delay in getting back to you, I now have further information to share with you in regards to the anti-Neuropeptide Y antibody (ab10980).

The antibody has been raised against the residues 10-45 of pig pro-neuropeptide. This matches to the region 29-64 of human pro-neuropeptide with a homology of 97% (only one residue difference). This region is the sequence which gets processed into the mature neuropeptide Y.

In your samples, you may therefore be detecting both the pro and mature neuropeptide. However, these wouldbe expected to be observed at ˜10 kDa for the full length pro-neuropeptide and at ˜4 kDa for the mature neuropeptide. As we have not performed Western blotting with this antibody ourselves we would not be able to ascertain if the results you have been seeing are what would be expected.

I have also had some difficulty in finding any references in literature where a similar experiment has been performed. I did however find reference to neuropeptide Y sometimes forming dimers. It may be that this is why you are observing bands at higher molecular weights than might be expected.

Are you performing the Western blot under reduced/denatuing conditions? How much protein have you been loading on the gel?

We would be very interested to be able to share your results with our other customers so that they can make an informed choice as to if they think it is worth trying in Western blotting. This can be done by filling in an Abreview. This should only take 5-10 minutes of your time and you will be rewarded with Abpoints which can be used to claim Amazon vouchers or credit off future Abcam orders. More information on the Abreview system can be found form the following link:

https://www.abcam.com/abreviews

I hope this information has been of some help. If you have any further questions, please do not hesitate to contact us again.

Read More

Answer

Thank you for contacting us.

I would like to share the reply I recieved from laboratory. "We have not tested productab10980 against the pro-peptide or in western blotting unfortunately. The antibody has been shown to cross-react with NPY (human), NPY (porcine), NPY (sheep), NPY (13-36), NPY (18-36), and PYY (human). It does not cross-react with neurokinin B (NKB), neurokinin A (NKA), substance P (SP), calcitonin, somatostatin and BSA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chicken brain cryosections)
Specification
Chicken brain cryosections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.1% Triton X-100
Blocking step
4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 02 2012

Answer

Thank you very much for your interest in our Neuropeptide Y antibodies.

As we have not tested any of these antibodies in western blot you are correct that they do not come under our Abpromsie, that does mean to say that they will not work, just that we have not tested them in western blot (unless it actually says on the website that it will not work in that application).

However in situations like these we can offer you our Testing discount as I think that ab10980 would work for you in western blot on mouse tissue and so I have placed details of how the testing discount program would work if you wanted to try ab10980 (or any other Neuropeptide Y antibody).

Testing Discount Details

As ab10980 has not been tested in western blot. Therefore, I can offer a discount off a future purchase if you buy ab10980 now, test it in western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab10980 in western blot. I will then send a discount code. This code must be issued before purchasing ab10980 so please wait for my reply before ordering.

2. Purchase ab10980 either by phone, fax, or online (www.abcam.com).

3. Test it in western blot.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10980 turns out to be unsuitable forwestern blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pediatric brain)
Specification
pediatric brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain, striatum)
Specification
brain, striatum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3 - 1.0% Trtion X100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Michael Craig

Verified customer

Submitted Dec 11 2007

Answer

The immunogen used was full-length neuropeptide Y. Based on this, the antibody could recognize either the N-terminal or the C-terminal. Please contact us again if you have any additional questions.

Read More

Answer

Thank you for your enquiry. I have been in touch with the source of this antibody and I have determined that 1:8000 is the correct dilution for IHC-P using this antibody. The antibody was validated using IHC-P; detected using a HRP-streptavidin system using DAB as the chromogenic detection method. The source of this antibody commented that whilst 1:8000 seems dilute, it is whole serum. Furthermore the antigen used to raise this antibody gave a very good response in the rabbit, hence the high titre. I would suggest that you apply the antibody in a dilution range akin to 1:8000 and determine the optimal dilution for your system. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. At this time, we are unaware of any publications that feature the use of ab10980 (the references listed on the online datasheet are just general / background references). Ab10980 has been tested for application in IHC-frozen sections and does cross-react with mouse. Please note that our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up