Overview

  • Product name

    Anti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description

    Rabbit polyclonal to Neuropeptide Y
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, ICC/IF, IHC-P, Dot blot, IHC-Fr, RIAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Monkey
  • Immunogen

    Full length Neuropeptide Y (porcine) conjugated to KLH.

  • Positive control

    • NPY-containing neurons (cell bodies, axons, and dendrites) in frozen sections of rat brain obtained following perfusion fixation with 4% paraformaldehyde. Formalin-fixed, frozen, or Vibratome sections of rat, cat, porcine, bovine, monkey, or human brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab10980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17455332Use at an assay dependent dilution. PMID 17455332.
ICC/IF Use at an assay dependent concentration. PubMed: 19609763
IHC-P 1/800.
Dot blot 1/10000. 1/10000. This concentration was determined using Neuropeptide Y-BSA (0.03-0.25 ng/dot).
IHC-Fr 1/8000. 1/8000. This concentration was determined using frozen sections of rat brain perfusion-fixed with 4% paraformaldehyde. Absorption studies show that specific staining of tissue may be inhibited following preincubation of the antiserum with 10 µM neuropeptide Y (porcine).
RIA 1/10000. 1/10000. This concentration was determined using 20 pg/tube of 125I-labeled neuropeptide Y in a second antibody and polyethylene glycol RIA. The sensitivity of the RIA assay is defined as the 90% intercept of a B/B0 standard curve. In this system the sensitivity has been found to be 20 pg/tube. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = Minimum 5.0 x 1010 L/mole

Target

Images

  • ab10980 staining rat brain, striatum tissue sections by IHC-P.  Sections were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% serum for 2 hours at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 14 hours at 20°C.  Arrows point to Neuropeptide Y positive interneurons. Magnification 20X.

    See Abreview

  • ab10980 staining Neuropeptide Y in chicken brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed with a Tris-EDTA buffer, pH 9. Samples were then permeabilized using 0.1% Triton X-100/PBS, blocked with 4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS for 1 hour at 4°C and then incubated with ab10980 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:

See all 16 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-4 of 4 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chicken brain cryosections)
Specification
Chicken brain cryosections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.1% Triton X-100
Blocking step
4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 02 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pediatric brain)
Specification
pediatric brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 21 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain, striatum)
Specification
brain, striatum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 08 2008

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3 - 1.0% Trtion X100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Michael Craig

Verified customer

Submitted Dec 11 2007

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