Key features and details
- Rabbit polyclonal to Neuropeptide Y
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Neuropeptide Y antibody
See all Neuropeptide Y primary antibodies
DescriptionRabbit polyclonal to Neuropeptide Y
Tested Applications & Species
Application Species IHC-PHuman
Synthetic peptide corresponding to Pig Neuropeptide Y aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionNPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Tissue specificityOne of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Sequence similaritiesBelongs to the NPY family.
- Information by UniProt
- C-flanking peptide of NPY antibody
- CPON antibody
- Neuropeptide tyrosine antibody
Immunohistochemistical detection of Neuropeptide Y using ab30914 on formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)
Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.
IHC image of Neuropeptide Y antibody staining in a section of formalin-fixed paraffin-embedded human cerebral cortex* tissue performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30914, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab30914 has been referenced in 48 publications.
- Cabral A et al. Fasting induces remodeling of the orexigenic projections from the arcuate nucleus to the hypothalamic paraventricular nucleus, in a growth hormone secretagogue receptor-dependent manner. Mol Metab 32:69-84 (2020). PubMed: 32029231
- Ghule A et al. Modulation of feeding behavior and metabolism by dynorphin. Sci Rep 10:3821 (2020). PubMed: 32123224
- Bentsen MA et al. Transcriptomic analysis links diverse hypothalamic cell types to fibroblast growth factor 1-induced sustained diabetes remission. Nat Commun 11:4458 (2020). PubMed: 32895383
- Cheng AH et al. SOX2-Dependent Transcription in Clock Neurons Promotes the Robustness of the Central Circadian Pacemaker. Cell Rep 26:3191-3202.e8 (2019). PubMed: 30893593
- Gonzalez-Reyes LE et al. Sonic Hedgehog is expressed by hilar mossy cells and regulates cellular survival and neurogenesis in the adult hippocampus. Sci Rep 9:17402 (2019). PubMed: 31758070