Anti-Neuropeptide Y antibody (ab30914)
- Datasheet
- References (18)
- Protocols
Overview
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Product nameAnti-Neuropeptide Y antibody
See all Neuropeptide Y primary antibodies -
DescriptionRabbit polyclonal to Neuropeptide Y
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Human, Common marmoset
Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.
Read Abcam's proprietary immunogen policy (Peptide available as ab32971.) -
Positive control
- IHC-P: Human cerebral cortex tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab30914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-FoFr | 1/3000. |
Target
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FunctionNPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
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Tissue specificityOne of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
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Sequence similaritiesBelongs to the NPY family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 396464 Chicken
- Entrez Gene: 504216 Cow
- Entrez Gene: 100724008 Guinea pig
- Entrez Gene: 4852 Human
- Entrez Gene: 109648 Mouse
- Entrez Gene: 397304 Pig
- Entrez Gene: 24604 Rat
- Entrez Gene: 574114 Rhesus monkey
see all -
Alternative names
- C-flanking peptide of NPY antibody
- CPON antibody
- Neuropeptide tyrosine antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Neuropeptide Y using ab30914 on formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an Abreview submitted by Dr Sophie Pezetab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA; tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.
Protocols
References
This product has been referenced in:
- Ogawa K et al. Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. Sci Rep 8:3615 (2018). Read more (PubMed: 29483626) »
- Gao Y et al. Deficiency of leptin receptor in myeloid cells disrupts hypothalamic metabolic circuits and causes body weight increase. Mol Metab 7:155-160 (2018). IHC-P ; Mouse . Read more (PubMed: 29174000) »
