Anti-Neuropeptide Y antibody (ab30914)

Rabbit polyclonal Neuropeptide Y antibody. Validated in IHC and tested in Mouse, Rat, Human, Common marmoset. Cited in 26 publication(s). Independently reviewed in 15 review(s).

Overview

  • Product name
    Anti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description
    Rabbit polyclonal to Neuropeptide Y
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Common marmoset
    Predicted to work with: Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.

    Read Abcam's proprietary immunogen policy (Peptide available as ab32971.)

  • Positive control
    • IHC-P: Human cerebral cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab30914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/3000.

Target

Images

  • Immunohistochemistical detection of Neuropeptide Y using ab30914 on  formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)

    See Abreview

  • Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None.  Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.

    See Abreview

  • IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA;  tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.

    See Abreview

References

This product has been referenced in:
  • Lepiarczyk E  et al. A study on preganglionic connections and possible viscerofugal projections from urinary bladder intramural ganglia to the caudal mesenteric ganglion in the pig. J Anat 234:263-273 (2019). Read more (PubMed: 30468248) »
  • Zhang M  et al. Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30411499) »
See all 30 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Unfortunately, we have not tested the cross reactivity of ab30914 against mouse PYY. With 69.4% homology with the immunogen we would not predict cross reactivity with mouse PYY.

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Answer

Thank you for contacting us and your interest in our products.

The immunogen sequence used to raise the anti-neuropeptide Y antibody (ab30914) is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly for your own use. The peptide sequence used is as follows:

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I have conducted some sequence alignments between this immunogen and the pancreatic polypeptide and PYY. For the pancreatic polypeptide, there is a 47% sequence homology and for the PYY there is ˜66-69% depending on the species. Therefore, it is unlikely that this antibody cross-reacts with either of these proteins and it is likely that the proteins that the antibody is detecting in your experiments is NPY.

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti Orexin ab35337, ab6214, ab16787, ab64565 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti leptin ab20101, ab20115, ab9749 and ab2095 Anti Insulin ab6995, ab63820, ab7760 and ab46707 Anti neuropeptide Y We ab30914, ab10341, ab6173, ab43871, ab53478. These antibodies are not yet tested in ELISA. Anti peptide YY  ab51537, ab51539 etc. Anti ACTH  ab20358, ab21003, ab104768,a b104619 Anti MCH ab37337; These are however not yet tested in ELISA. Please check the datasheets of these antibodies for more up-to-date information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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