Recombinant
RabMAb

Recombinant Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)

Overview

  • Product name

    Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free
    See all Neuropeptide Y primary antibodies
  • Description

    Rabbit monoclonal [EPR21877] to Neuropeptide Y - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Neuropeptide Y aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P01303

  • Positive control

    • IHC-P: Human pheochromocytoma tissue.
  • General notes

    Ab234527 is the carrier-free version of ab221145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

1:2000 dilution recommended for human, mouse and rat species.
1:10000 dilution recommended for mouse adrenal gland tissue.

IHC-Fr Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in chromaffin cells of rat adrenal gland medulla (PMID: 15623622) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
    Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

  • Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on chromaffin cells of mouse adrenal gland medulla (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
    Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse cerebrum (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
    Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tisssue labeling Neuropeptide Y with ab221145 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining in hypothalamic paraventricular nucleus of rat cerebrum (PMID: 19357287) is observed. Counterstained with DAPI (Nuclear).

    Perform heat mediated antigen retrieval with sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

  • Immunohistochemical analysis of paraffin-embedded human pheochromocytoma tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in human pheochromocytoma (PMID: 15623622) is obserevd. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retreival.
    Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

     

References

ab234527 has not yet been referenced specifically in any publications.

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