Anti-Neuropilin 1 antibody (Biotin) (ab83435)
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Overview
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Product name
Anti-Neuropilin 1 antibody (Biotin)
See all Neuropilin 1 primary antibodies -
Description
Goat polyclonal to Neuropilin 1 (Biotin) -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant human Neuropilin 1
Properties
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Form
Lyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab83435 is sterile filtered. This antibody was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab83435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Neuropilin 1. | |
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Neuropilin 1 (ab101470). When used as detection antibody paired with recommended antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Neuropilin 1. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 103 kDa. The detection limit for recombinant human Neuropilin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. -
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
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Alternative names
- A5 protein antibody
- BDCA4 antibody
- BLOOD DENDRITIC CELL ANTIGEN 4 antibody
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Protocols
Datasheets and documents
References
ab83435 has not yet been referenced specifically in any publications.
