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  1. Link

    neuropilin-1-antibody-biotin-ab83435.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-Neuropilin 1 antibody (Biotin) (ab83435)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Neuropilin 1 antibody (Biotin)
    See all Neuropilin 1 primary antibodies
  • Description

    Goat polyclonal to Neuropilin 1 (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human Neuropilin 1

Properties

  • Form

    Lyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab83435 is sterile filtered. This antibody was purified by affinity chromatography and then biotinylated.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Isotype control

    • Goat IgG Biotin - Isotype Control (ab37376)
  • Recombinant Protein

    • Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)
  • sELISA pair antibody

    • Anti-Neuropilin 1 antibody (ab101470)

Applications

Our Abpromise guarantee covers the use of ab83435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Neuropilin 1.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Neuropilin 1 (ab101470). When used as detection antibody paired with recommended antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Neuropilin 1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 103 kDa. The detection limit for recombinant human Neuropilin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession O14786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8829 Human
    • Omim: 602069 Human
    • SwissProt: O14786 Human
    • Unigene: 131704 Human
    • Unigene: 653996 Human
    • Alternative names

      • A5 protein antibody
      • BDCA4 antibody
      • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
      • CD304 antibody
      • Neuropilin-1 antibody
      • Neuropilin1 antibody
      • NP1 antibody
      • NPN1 antibody
      • NRP 1 antibody
      • NRP antibody
      • NRP1 antibody
      • NRP1_HUMAN antibody
      • transmembrane receptor antibody
      • Vascular endothelial cell growth factor 165 receptor antibody
      • VEGF165R antibody
      see all

    Protocols

    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab83435 has not yet been referenced specifically in any publications.

    Publishing research using ab83435? Please let us know so that we can cite the reference in this datasheet.

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