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RecombinantRabMAb

Recombinant Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

  • Datasheet
  • SDS
Reviews (31)Q&A (4)References (78)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

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Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
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Overview

  • Product name

    Anti-Neuropilin 1 antibody [EPR3113]
    See all Neuropilin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Neuropilin 1 aa 900 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: O14786
    (Peptide available as ab189308)

  • Positive control

    • WB: HUVEC and HepG2 whole cell lysate (ab7900), human placenta, kidney and heart, mouse heart and kidney and rat heart and kidney tissue lysates. IHC-P: Human liver tissue; Rat brain tissue; Mouse brain tissue. ICC: MCF7 and HUVEC cells; Omentum and effluent-derived mesothelial cells; COS1 fibroblast-like cell line derived from monkey kidney tissue. Flow Cyt: HepG2 and MCF7 cells. IHC-Fr: Human kidney tissue. IP: Mouse heart tissue lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3113
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    • Alexa Fluor® 488 Anti-Neuropilin 1 antibody [EPR3113] (ab197644)
    • Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
    • PE Anti-Neuropilin 1 antibody [EPR3113] (ab209445)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunizing Peptide (Blocking)

    • Neuropilin 1 peptide (ab189308)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
    • Mouse heart tissue lysate - total protein (7 days) (ab7196)
    • Mouse kidney tissue lysate - total protein (0 days) (ab7261)
    • Mouse kidney tissue lysate (14 days) (ab7262)
    • Mouse kidney tissue lysate (7 days) (ab7263)
  • Recombinant Protein

    • Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
IP
Mouse
WB
Human
All applications
Monkey
Common marmoset
Application Abreviews Notes
ICC
1/250.
WB (6)
1/1000 - 1/2000. Predicted molecular weight: 103 kDa.Can be blocked with Neuropilin 1 peptide (ab189308).
IHC-P (8)
1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (1)
1/50 - 1/70.

The epitope that the antibody recognizes is intracellular. Fixation and permeabilization are necessary.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/30.
Notes
ICC
1/250.
WB
1/1000 - 1/2000. Predicted molecular weight: 103 kDa.Can be blocked with Neuropilin 1 peptide (ab189308).
IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/50 - 1/70.

The epitope that the antibody recognizes is intracellular. Fixation and permeabilization are necessary.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/30.

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession O14786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8829 Human
    • Entrez Gene: 18186 Mouse
    • Entrez Gene: 246331 Rat
    • Omim: 602069 Human
    • SwissProt: O14786 Human
    • SwissProt: P97333 Mouse
    • SwissProt: Q9QWJ9 Rat
    • Unigene: 131704 Human
    • Unigene: 653996 Human
    • Unigene: 271745 Mouse
    • Unigene: 10815 Rat
    see all
  • Alternative names

    • A5 protein antibody
    • BDCA4 antibody
    • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Neuropilin 1 with purified ab81321 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)Image from Pérez-Lozano ML et al., PLoS One. 2013;8(4):e60776. Fig 5.; doi: 10.1371/journal.pone.0060776.

    The expression of Neuropilin 1, VEGFR-2, and VEGF was analyzed by immunofluorescence microscopy in omentum and effluent-derived mesothelial cells (MCs). MCs were double stained for Neuropilin 1 (green) and VEGFR-2 (red), and single stained for VEGF (green). Nuclei were stained with DAPI. Neuropilin 1 and VEGF show a membrane distribution in omentum and epithelioid MCs (b, c, h, i). During in vitro (e, f) and ex vivo (k, l) MMT both proteins change their localization and are internalized. The expression of VEGFR-2 is down-regulated but it does not show differences in localization during in vitro (a, d) and ex vivo (g, j) MMT.

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/10000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Flow Cytometry analysis of MCF7 cells labelling Neuropilin 1 with purified ab81321 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Neuropilin 1 was immunoprecipitated from 0.35mg mouse heart lysate with ab81321 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab81321 at 1/1000 dilution (0.77 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: Mouse heart tissue lysate 10 μg
    Lane 2: Mouse heart tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab81321 in mouse heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/10000 dilution (purified) + Human heart tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    ICC/IF image of unpurified ab81321 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab81321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Flow Cytometry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Overlay histogram showing HepG2 cells stained with unpurified ab81321 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab81321, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/2000 dilution (purified) + Human placenta tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling Neuropilin 1 with purified ab81321 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (78)

    Publishing research using ab81321? Please let us know so that we can cite the reference in this datasheet.

    ab81321 has been referenced in 78 publications.

    • Zhu Q  et al. Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor microenvironment. Biol Res 53:5 (2020). PubMed: 32046779
    • Kutikhin AG  et al. Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature. Cells 9:N/A (2020). PubMed: 32260159
    • Cantuti-Castelvetri L  et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science 370:856-860 (2020). IHC-P, ICC/IF ; Mouse . PubMed: 33082293
    • Daly JL  et al. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science 370:861-865 (2020). PubMed: 33082294
    • Mei B  et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 35 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E14.5 Mouse Embryo (Sagittal section))
    Permeabilization
    Yes - Triton
    Specification
    E14.5 Mouse Embryo (Sagittal section)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Alain Chedotal

    Verified customer

    Submitted Feb 25 2021

    IHC - Wholemount abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E12.5 Mouse Embryo)
    Specification
    E12.5 Mouse Embryo
    Read More

    Dr. Alain Chedotal

    Verified customer

    Submitted Feb 25 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Rat bearing rat brain tumor 9L)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 0.2%
    Specification
    Rat bearing rat brain tumor 9L
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Jun 03 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Human Tissue sections (Mouse bearing human tumor U87)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate 10 mM
    Permeabilization
    Yes - triton 1%
    Specification
    Mouse bearing human tumor U87
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Apr 30 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Mouse bearing Mouse brain tumor GL261)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate 10mM
    Permeabilization
    Yes - triton 1%
    Specification
    Mouse bearing Mouse brain tumor GL261
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Apr 30 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Brain cell culture)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Brain cell culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain cell culture)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Brain cell culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 18 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
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    Verified customer

    Submitted Feb 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Brain
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2020

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