Immunohistochemistry (Frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain section)
Specification
Brain section
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% serum in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Cy3-AffiniPure Anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
Antigen retrival was done using 10 mM Citrate buffer (pH 6.0) . Upon permeabilisation with 0.3% triton X-100, unspecific binding is observed.

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Submitted Sep 16 2010

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