Western blot abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
30 µg
Specification
HUVEC
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 120 kDa

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Submitted Feb 28 2013

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