Application
Western blot
Sample
Rabbit Tissue lysate - whole (Heart)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
12 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA-PBS-Tween (0.1%)
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabb
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 120 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 25 2019