Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain cell culture)
Permeabilization
Yes - 0.05% tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
The signal obtained from using Anti-Neuropilin 1 antibody on a mouse brain culture was pseudo colored in Green and the nuclei stained Blue with Hoechst . Alexa Fluor 647 secondary was used for its better resolution and lacking autofluorescence.
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Submitted Feb 18 2020