Immunohistochemistry (PFA perfusion fixed frozen sections)
Human Tissue sections (Mouse bearing human tumor U87)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10 mM
Yes - triton 1%
Mouse bearing human tumor U87
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Other product details
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: dk serum 5%
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed
Abcam has not tested ab81321 in IHC-FoFr and thus could not guarantee the antibody performance in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Submitted Apr 30 2020