Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Rat bearing rat brain tumor 9L)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
Rat bearing rat brain tumor 9L
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Dk serum 5%
Dilution
1/500
Secondary antibody
Dilution
1/250
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Verified customer
Submitted Jun 03 2020